1887

Abstract

SUMMARY In some nisin-producing strains of , clones which were permanently incapable of producing nisin (Nis) appeared spontaneously with a frequency of 0·03 to 0·82 %. Treatment with proflavin (Pro), ethidium bromide (EB) or at a temperature of 40 °C increased the number of such clones. In one strain 78 % of cells became Nis after treatment with Pro.

Treatment with nitrosoguanidine did not reverse the effect of Pro or EB. There was no evidence that Pro or EB selected spontaneously-occurring Nis clones.

These observations suggest that the gene(s) responsible for nisin production in some strains may be located on a plasmid, but further investigation is required to confirm this.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-83-2-295
1974-08-01
2021-07-30
Loading full text...

Full text loading...

/deliver/fulltext/micro/83/2/mic-83-2-295.html?itemId=/content/journal/micro/10.1099/00221287-83-2-295&mimeType=html&fmt=ahah

References

  1. Courvalin P. M., Carlier C., Chabbert Y. A. 1972; Plasmid-linked tetracycline and erythromycin resistance in group D Streptococcus. Annales de l’Institut Pasteur 123:755–759
    [Google Scholar]
  2. Hurst A. 1966; Biosynthesis of the antibiotic nisin by whole Streptococcus lactis organisms. Journal of General Microbiology 44:209–220
    [Google Scholar]
  3. Hurst A., Paterson G. M. 1971; Observations on the conversion of an inactive precursor protein to the antibiotic nisin. Canadian Journal of Microbiology 17:1370–1384
    [Google Scholar]
  4. Kozak W., Rajchert-Trzpil M., Zajdel J., Dobrzański W. T. 1973; Lysogeny in lactic Streptococci producing and not producing nisin. Applied Microbiology 25:305–308
    [Google Scholar]
  5. Mattick A. T. R., Hirsch A. 1944; A powerful inhibitory substance produced by group N streptococci. Nature; London: 154551–552
    [Google Scholar]
  6. Mckay L. L., Cords B. R., Baldwin K.A. 1973; Transduction of lactose metabolism in Streptococcus lactis C2. Journal of Bacteriology 115:810–815
    [Google Scholar]
  7. Novick R. P. 1969; Extrachromosomal inheritance in bacteria. Bacteriological Reviews 33:210–263
    [Google Scholar]
  8. Okanishi M., Ohta T., Umezawa H. 1970; Possible control of formation of aerial mycelium and antibiotic production in Streptomyces by episomic factors. The Journal of Antibiotics 23:45–47
    [Google Scholar]
  9. Riva S., Fietta A. M., Silvestri L. G., Romero E. 1972; Effect of rifampicin on expression of some episomal genes in E. coli . Nature New Biology 235:78–80
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-83-2-295
Loading
/content/journal/micro/10.1099/00221287-83-2-295
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error