1887

Abstract

Summary: Intraspecific transformation in three strains of was observed with respect to gelatin liquefaction and chlortetracycline resistance. In interspecific transformation experiments involving 23 pairs of strains and four markers, two yielded positive results: a strain of acquired the property of gelatin liquefaction when transformed with DNA, and a strain of fish Pseudomonas, incapable of growing at 37 °C, did so when transformed with DNA from another strain of . The guanine-cytosine content of the DNA of the strains investigated ranged between 61·0 and 64·4 mol %. The transforming ability of DNA was destroyed by DNase. The divalent ions Mg, Ca and Ba improved transformation frequency several fold. Actinomycin D inhibited transformation.

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/content/journal/micro/10.1099/00221287-83-2-251
1974-08-01
2021-08-05
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References

  1. Balassa G. 1963; Genetic transformation of RFizobium: A review of the work of R. Balassa. Bacteriological Review 27:228–244
    [Google Scholar]
  2. Kammen H. C., Wajner R. J., Canellakis E. S. 1966; Transformation in Bacillus subtilis. II. The development and maintenance of the competent state. Biochimica et biophysica acta 123:56–65
    [Google Scholar]
  3. Khan N. C., Sen S. P. 1967; Genetic transformation in Pseudomonae. Journal of General Microbiology 49:201–209
    [Google Scholar]
  4. Leonard C. G., Matheis D. K., Matheis M. J., Housewright R. D. 1964; Transformation to prototrophy and polyglutamic acid synthesis in Bacillus licheniformis. Journal of Bacteriology 88:220–225
    [Google Scholar]
  5. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  6. Morihara K. 1964; Production of elastase and proteinase by Pseudomonas aeruginosa. Journal of Bacteriology 88:745–757
    [Google Scholar]
  7. Palleroni N. J., Ballard R. W., Ralston E., Doudoroff M. 1972; DNA homologies among some Pseudomonas species. Journal of Bacteriology no:1–11
    [Google Scholar]
  8. Spizizen J., Reilly B. E., Evans A. H. 1966; Microbial transformation and transfection. Annual Review of Microbiology 22:371–400
    [Google Scholar]
  9. Suzuki K., Yamagami H., Shimazu Y. 1965; Effect of mitomycin C on early phenotypic expression in the transformation of Diplococcos pneumoniae. Nature; London: 205929–930
    [Google Scholar]
  10. Young F. F., Spizizen J. 1961; Physiological and genetic factors affecting transformation of Bacillus subtilis. Journal of Bacteriology 81:823–829
    [Google Scholar]
  11. Young F. F., Spizizen J. 1963; Incorporation of deoxyribonucleic acid in the Bacillus subtilis transformation system. Journal of Bacteriology 86:392–400
    [Google Scholar]
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