1887

Abstract

SUMMARY can obtain energy and required nutrients by eating several different bacteria or brewer’s yeast. The phagocytic process includes aggregation of bacteria into clumps which contain and are surrounded by Ochromonas.

Bacteria were phagocytized too fast to be seen while yeast took at least 5 min to be engulfed. 2,4-Dinitrophenol or sodium azide, but not sodium fluoride or iodo-acetic acid, inhibited the phagocytic process; aerobic energy metabolism was necessary for phagocytosis. Food organisms were engulfed and the food vacuole migrated to the posterior end of the organism behind the leucosin vacuole. The bacteria were digested mainly in the food vacuole although secondary lysosomes were formed. Digestion of bacteria resulted in the proliferation of vesicles and membranes from the food vacuole membrane and the proliferation of membrane from the bacteria being digested.

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/content/journal/micro/10.1099/00221287-83-1-21
1974-07-01
2022-01-20
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References

  1. Aaronson A. 1971; The synthesis of extracellular molecules and membranes by a population of the phytoflagellate Ochromonas danica . Limnology and Oceanography 16:1–9
    [Google Scholar]
  2. Aaronson S. 1972; A new source of single-cell protein and other nutrients. Archiv für Hydrobiologie 41:108–115
    [Google Scholar]
  3. Aaronson S. 1973a; Digestion in flagellates. In Lysosomes in Biology and Medicine 3 pp 18–27 Dingle J. T. Edited by Amsterdam: North-Holland Publishing;
    [Google Scholar]
  4. Aaronson S. 1973b; Particle aggregation and phagotrophy by Ochromonas . Archiv für Mikrohiologie 92:39–44
    [Google Scholar]
  5. Aaronson S., Baker H. 1959; A comparative biochemical study of two species of Ochromonas . Journal of Protozoology 6:282–284
    [Google Scholar]
  6. Aaronson S., Behrens U., Orner R., Haines T. H. 1971; Ultrastructure of intracellular and extracellular vesicles, membranes, and myelin figures produced by Ochromonas danica . Journal of Ultrastructure Research 35:418–430
    [Google Scholar]
  7. Chapman-Andresen C. 1971; Biology of the large amoebae. Annual Review of Microbiology 25:27–48
    [Google Scholar]
  8. Daley R. J., Morris G. P., Brown S. R. 1973; Phagotrophic ingestion of a blue-green alga by Ochromonas . Journal of Protozoology 20:58–61
    [Google Scholar]
  9. Elliott A. M., Clemmons G. L. 1966; An ultrastructural study of ingestion and digestion in Tetra- hymena pyriformis . Journal of Protozoology 13:311–323
    [Google Scholar]
  10. Gezelius K. 1959; The ultrastructure of cells and cellulose membranes in acrasiae. Experimental Cell Research 18:425–453
    [Google Scholar]
  11. Gezelius K. 1961; Further studies in the ultrastructure of acrasiae. Experimental Cell Research 23:300–310
    [Google Scholar]
  12. Hall R. P. 1965 Protozoan Nutrition pp 31–32 New York: Blaisdell Publishing Co;
    [Google Scholar]
  13. Hardin G. 1943; Flocculation of bacteria by protozoa. Nature; London: 151642
    [Google Scholar]
  14. Hill D. L. 1972 The Biochemistry and Physiology of Tetrahymena p 14 New York: Academic Press;
    [Google Scholar]
  15. Mercer E. H., Shaffer B. M. 1960; Electron microscopy of solitary and aggregated slime mold cells. Journal of Biophysical and Biochemical Cytology 7:353–356
    [Google Scholar]
  16. Müller M. 1967; Digestion. In Chemical Zoology 1The Protozoa pp 351–380 Florkin M., Scheer B. T. Edited by New York: Academic Press;
    [Google Scholar]
  17. Nilsson J. R. 1972; Further studies on vacuole formation in Tetrahymenapyriformis gl . Compte rendu des travaux du Laboratoire Carlsberg 39:83–110
    [Google Scholar]
  18. Pearsall N. N., Weiser R. S. 1970 The Macrophage pp 42–43 Philadelphia: Lea & Febiger;
    [Google Scholar]
  19. Pitelka D. R. 1963 Electron-microscopic Structure of Protozoa Oxford: Pergamon Press;
    [Google Scholar]
  20. Pringsheim E. G. 1952; On the nutrition of Ochromonas . Quarterly Journal of Microscopical Science 93:71–96
    [Google Scholar]
  21. Pringsheim E. G. 1955; Über Ochromonas danica n. sp. und andere Arten der Gattung. Archiv für Mikrobio/ogie 23:181–192
    [Google Scholar]
  22. Rabinovitch M., DeStefano M. J. 1971; Phagocytosis of erythrocytes by Acanthamoeba sp. Experimental Cell Research 64:275–284
    [Google Scholar]
  23. Rapport D. J., Berger J., Reid D. B. W. 1972; Determination of food preference of Stentor coeruleus . Biological Bulletin 142:103–109
    [Google Scholar]
  24. Ricketts T. R. 1970; Effect of endocytosis upon acid phosphatase activity of Tetrahymena pyriformis . Protoplasma 71:127–137
    [Google Scholar]
  25. Ricketts T. R. 1971a; Periodicity of endocytosis in Tetrahymena pyriformis . Protoplasma 73:387–396
    [Google Scholar]
  26. Ricketts T. R. 1971b; Endocytosis in Tetrahymena pyriformis . Experimental Cell Research 66:49–58
    [Google Scholar]
  27. Rosenbaum R. M., Wittner M. 1970; Ultrastructure of bacterized and axenic trophozoites of Entamoeba histolytica with particular reference to helical bodies. Journal of Cell Biology 45:367–382
    [Google Scholar]
  28. Roth L. E. 1960; Electron microscopy of pinocytosis and food vacuoles in Pelomyxa . Journal of Protozoology 7:176–185
    [Google Scholar]
  29. Rudzinska M. A. 1970; The mechanism of food intake in Tokophrya infusionum and ultrastructural changes in food vacuoles during digestion. Journal of Protozoology 17:626–641
    [Google Scholar]
  30. Rudzinska M. A., Jackson G. J., Tuffran M. 1966; The fine structure of Colpoda maupasi with special emphasis on food vacuoles. Journal of Protozoology 13:440–459
    [Google Scholar]
  31. Schuster F. 1963; An electron microscope study of the amoeboflagellate, Naegleria griiberi (Schardinger). I. The amoeboid and flagellate stages. Journal of Protozoology 10:297–313
    [Google Scholar]
  32. Schuster F. 1964; Electron microscope observations on spore formation in the true slime mold Didymium nigripes . Journal of Protozoology 11:207–216
    [Google Scholar]
  33. Schuster F. L., Hershenov B., Aaronson S. 1968; Ultrastructural observations on aging of stationary cultures and feeding in Ochromonas . Journal of Protozoology 15:335–346
    [Google Scholar]
  34. Spies F., Elbers P. F. 1972; Axenic mass cultivation of Entamoeba invadens and cell membrane isolation. Journal of Protozoology 19:102–107
    [Google Scholar]
  35. Vickerman K. 1960; Patterns of cellular organization in Umax amoebae. Experimental Cell Research 26:497–519
    [Google Scholar]
  36. Weisman R. A., Korn E. D. 1967; Phagocytosis of latex beads by Acanthamoeba. 1. Biochemical properties. Biochemistry 6:485–497
    [Google Scholar]
  37. Weisman R. A., Moore M. O. 1969; Bead uptake as a tool for studying differentiation in Acanthamoeba . Experimental Cell Research 54:17–22
    [Google Scholar]
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