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Summary: In cell-free extracts, pyruvate, formate, malate and NADPH provided reducing equivalents to the nitrogenase of the nitrogen-fixing colform, Klebsiella pnemoniae. Two electron carriers were isolated from extracts, both capable of mediating the transfer of reducing power between illuminated spinach chloroplasts and the nitrogenases of Klebsiella or Azotobacter vinelandii. One electron carrier was a flavoprotein, named Klebsiella flavodoxin (molecular weight about 21000); the second was not characterized. A similarity is suggested between Klebsiella and Escherichia coli in the generation and transport of low-potential reducing power from pyruvate.
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