1887

Microbiology Society logo

Volume 83, Issue 1

Scanning Electron Microscopic Studies on the Growth Features of in Cell-free Medium Free

Abstract

SUMMARY: The morphology of Hawaiian strain, cultivated on a glass slide in a cell-free liquid medium (NC5) at 30 °C for 12 weeks, was observed every 2 weeks with a scanning electron microscope. Flourishing growth and microcolonial growth occurred in the peripheral and central areas, respectively, of a smear. Elongation, septum formation, division, budding and branching in the bacteria were seen between the second and fourth weeks of incubation, which may correspond to the beginning of the exponential phase. After this period, the number of bacilli suddenly increased, and intertwined, elongated cells with large granules appeared. These features gradually increased until the stationary phase was reached after 12 weeks of incubation. These observations are the first scanning electron microscopical descriptions of the growth patterns of .

  • Received:
  • Published Online:
© Society for General Microbiology 1974
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-83-1-145
1974-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/83/1/mic-83-1-145.html?itemId=/content/journal/micro/10.1099/00221287-83-1-145&mimeType=html&fmt=ahah

References

  1. Kirchner O. 1932; Die Leistungsfähigkeit der Tiefenkultur des Tuberkelbazillus bei Verwendung besonders geeigneter fliissiger Nährboden. Zeniralblatt für Bakteriologie, Parasitenkunde, Infektions-krankheiten und Hygiene Abteilung I 124:403–412
     [Google Scholar]
  2. Knaysi G. 1951; The spores of bacteria. In Elements of Bacterial Cytology pp 259–260 New York: Comstock;
     [Google Scholar]
  3. Nakamura M. 1972; Multiplication of Mycobacterium lepraemurium in cell-free medium containing α-ketoglutaric acid and cytochrome c . Journal of General Microbiology 73:193–195
     [Google Scholar]
  4. Nakamura M. 1973; Multiplication of Mycobacterium lepraemurium in a cell free liquid medium. Proceedings of the Japan Academy 49:42–46
     [Google Scholar]
  5. Nakamura M. 1974; Quantitative multiplication of Mycobacterium lepraemurium in a cell-free liquid medium (NC-5). Journal of General Microbiology 82:385–391
     [Google Scholar]
  6. Penso G. 1964 In Atlas of Electron Microscopy, Biological Applications Scanga F. Edited by Amsterdam: Elsevier;
     [Google Scholar]
  7. Stefansky W. K. 1903; Eine lepra-ähnliche Erkrankung der Haut und der Lymphdrüsen bei Wanderratte. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene Abteilung I 33:481–487
     [Google Scholar]
  8. Stempen H., Hutchinson W. G. 1951a; The formation and development of large bodies in Proteus vulgaris ox-19. I. Bright phase contrast observations of living bacteria. Journal of Bacteriology 61:321–335
     [Google Scholar]
  9. Stempen H., Hutchinson W. G. 1951b; The formation and development of large bodies in Proteus vulgaris ox-19. II. Comparative cytology of bacilli and large bodies. Journal of Bacteriology 61:337–349
     [Google Scholar]
  10. Yoshii Z., Nakamura M. 1973; Scanning electron microscopy of Mjwbacteriim Iepruenittriunr culti- vated on the slide glass. Proceedings of the Jcipun Acucletriy 49:47–50
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-83-1-145
Loading
Scanning Electron Microscopic Studies on the Growth Features of Mycobacterium lepraemurium in Cell-free Medium
Microbiology 83, 145 (1974); https://doi.org/10.1099/00221287-83-1-145
/content/journal/micro/10.1099/00221287-83-1-145
/content/journal/micro/10.1099/00221287-83-1-145
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error