1887

Abstract

SUMMARY: Purified isoenzymes of α-L-arabinofuranosidase (AFI, pI value 3.0) and polygalacturonase (PGIII, pI 5–5; PGIV, pI 9.7) separated from culture filtrates of by isoelectric focusing and gel chromatography were used to produce antisera in sheep. The serum produced to AFI was specific as judged by immunodiffusion, immunoadsorbent column chromatography and binding of fluorescein-conjugated serum to the antigen separated by polyacrylamide gel electrophoresis. Fluorescein-labelled anti-AFI serum was bound to mycelium producing this isoenzyme in liquid culture, and binding was decreased when AF production was repressed with glucose; no labelling occurred on mycelium infecting apple fruit tissue.

Data for the anti-PG sera were less conclusive. The antigens gave precipitin lines only with homologous sera, but an anti-PG III immunoadsorbent column bound PG in as well as a smaller proportion of PG IV, while an anti-PG IV column bound neither antigen. Fluorescein isothiocyanate-labelled anti-PG III serum was bound to mycelium of grown in liquid culture and to mycelium-infected apple fruit tissue, but labelled anti-PG IV serum was not bound.

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/content/journal/micro/10.1099/00221287-83-1-135
1974-07-01
2020-01-25
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