1887

Abstract

SUMMARY: A methane-oxidizing bacterium, isolated from soil, was capable of fixing nitrogen. Nitrogenase activity could be assayed by acetylene reduction when the bacterium was growing on methanol but not when growing on methane, although N was fixed. Bacteria growing on methane co-oxidized ethylene but methanolgrowing cells did not. The organism was extremely sensitive to oxygen when dependent on N as nitrogen source, a consequence of the sensitivity of its nitrogenase towards oxygen.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-83-1-113
1974-07-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/83/1/mic-83-1-113.html?itemId=/content/journal/micro/10.1099/00221287-83-1-113&mimeType=html&fmt=ahah

References

  1. Adamse A. D., Hoeks J., De Bont J. A. M., Van Kessel J. F. 1971; Microbial activities in soil near natural gas leaks. Archiv für Mikrobiologie 83:32–51
    [Google Scholar]
  2. Akkermans A. D. L. 1971 Nitrogen fixation and nodulation of Alnus and Hippophae under natural conditions Thesis University of Leiden, The Netherlands:
    [Google Scholar]
  3. Coty V. F. 1967; Atmospheric nitrogen fixation by hydrocarbon-oxidizing bacteria. Biotechnology and Bioengineering 9:25–32
    [Google Scholar]
  4. Dalton H., Postgate J. R. 1969; Effect of oxygen on growth of Azotobacter chroococcum in batch and continuous cultures. Journal of General Microbiology 54:463–473
    [Google Scholar]
  5. Davis J. B., Coty V. F., Stanley J. P. 1964; Atmospheric nitrogen fixation by methane-oxidizing bacteria. Journal of Bacteriology 88:468–472
    [Google Scholar]
  6. Drozd J., Postgate J. R. 1970; Interference by oxygen in the acetylene-reduction test for aerobic nitrogen-fixing bacteria. Journal of General Microbiology 60:427–429
    [Google Scholar]
  7. Faust H. 1967; Probenchemie 15N markierter Stickstoffverbindungen im Mikro- bis Nanomolbereich fur die emissionsspektrometrische Isotopenanalyse. Isotopenpraxis 3:100–103
    [Google Scholar]
  8. Ferraris M. M., Proksch G. 1972; Calibration methods and instrumentation for optical 15N determinations with electrodeless discharge tubes. Analytica chimica acta 59:177–185
    [Google Scholar]
  9. Hardy R. W. F., Burns R. C., Holsten R. D. 1973; Applications of the acetylene-ethylene assay for measurement of nitrogen fixation. Soil Biology and Biochemistry 5:47–81
    [Google Scholar]
  10. Harper H. J. 1939; The effect of natural gas on the growth of micro-organisms and the accumulation of nitrogen and organic matter in the soil. Soil Science 48:461–466
    [Google Scholar]
  11. Hazeu W., Steennis P. J. 1970; Isolation and characterization of two vibrio-shaped methane-oxidizing bacteria. Antonie van Leeuwenhoek 36:67–72
    [Google Scholar]
  12. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. In Methods in Microbiology 5B pp 209–344 London and New York: Academic Press;
    [Google Scholar]
  13. Hill S. 1971; Influence of oxygen concentrations on the colony type of Derxia gummosa grown on nitrogen- free media. Journal of General Microbiology 67:77–83
    [Google Scholar]
  14. Large P. J., Quayle J. R. 1962; Microbial growth on C1 compounds. V. Enzyme activities in extracts of Pseudomonas AMI. Biochemical Journal 87:386–396
    [Google Scholar]
  15. Lawrence A. J., Quayle J. R. 1970; Alternative carbon assimilation pathways in methane-utilizing bacteria. Journal of General Microbiology 63:371–374
    [Google Scholar]
  16. Namsaraev B. B., Zavarzin T. A. 1972; Trophic relationships in a methane-oxidizing culture. In Mikrobiologiya 41 pp 999–1006 English translation pp 887–892 New York: Plenum Publishing Corp;
    [Google Scholar]
  17. Parejko R. A., Wilson P. W. 1971; Kinetic studies on Klebsiella pneumoniae nitrogenase. Proceedings of the National Academy of Sciences of the United States of America 68:2016–2018
    [Google Scholar]
  18. Postgate R. 1971; Biochemical and physiological studies with free-living, nitrogen-fixing bacteria. Plant and Soil, Special 1971551–559
    [Google Scholar]
  19. Quayle J. R. 1972; The metabolism of one-carbon compounds by microorganisms. Advances in Microbial Physiology 7:119–203
    [Google Scholar]
  20. Schollenberger C. J. 1930; Effect of leaking natural gas upon the soil. Soil Science 29:261–266
    [Google Scholar]
  21. Whittenbury R., Phillips K. C., Wilkinson J. F. 1970; Enrichment, isolation and some properties of methane-utilizing bacteria. Journal of General Microbiology 61:205–218
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-83-1-113
Loading
/content/journal/micro/10.1099/00221287-83-1-113
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error