1887

Abstract

SUMMARY: Mutants of 2 requiring acetate or succinate for aerobic growth on glucose were isolated. One acetate-requiring mutant, s8, lacked the activity of the overall pyruvate dehydrogenase complex due to a deficiency in the pyruvate dehydrogenase component (E1p) and was therefore designated an mutant. Another mutant, s6, which required succinate (or lysine plus methionine), lacked activities of the overall α-ketoglutarate dehydrogenase complex and the α-ketoglutarate dehydrogenase component (E1 kg) and was designated a mutant. Genetic studies with these mutants established that like 12, 2 has an gene linked to and thus: and a gene linked to thus:

Two deletion strains, 16 and 51, which required acetate, or better, acetate plus lysine plus methionine, were found to lack the overall activities of both α-keto acid dehydrogenase complexes. Biochemical and immunological studies showed this to be due to deficiences in pyruvate dehydrogenase and dihydrolipoyl- transacetylase (the E1p and E2p components of the pyruvate complex) and failure to synthesize lipoamide dehydrogenase (the E3 components of both complexes). These deletion strains also behaved as if they lacked the general aromatic permease (), and 51, which requires nicotinate, was probably deleted for the gene. Genetic studies confirmed that these strains were deleted in the region. The results also indicated that the gene-protein- relationships of the α-keto acid dehydrogenase complexes are similar in and

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-82-2-319
1974-06-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/82/2/mic-82-2-319.html?itemId=/content/journal/micro/10.1099/00221287-82-2-319&mimeType=html&fmt=ahah

References

  1. Alwine J. C., Russell F. M., Murray K. N. 1973; Characterization of an Escherichia coli mutant deficient in dihydrolipoyl dehydrogenase activity. Journal of Bacteriology 115:1–8
    [Google Scholar]
  2. Ames G. F., Roth J. R. 1968; Hisitidine and aromatic permeases of Salmonella typhimurium . Journal of Bacteriology 98:1742–1749
    [Google Scholar]
  3. Brown K. D. 1970; Formation of aromatic amino acid pools in Escherichia coli k-12. Journal of Bacteriology 104:177–188
    [Google Scholar]
  4. Carrillo-Castaneda G., Ortega M. V. 1970; Mutants of Salmonella typhimurium lacking phos- phoe/20/pyruvate carboxykinase and a-ketoglutarate dehydrogenase activities. Journal of Bacteriology ioa:524–530
    [Google Scholar]
  5. Clowes R. C. 1972; Molecular structure of bacterial plasmids. Bacteriological Reviews 36:361–405
    [Google Scholar]
  6. Creaghan I. T., Guest J. R. 1972; Amber mutants of the a-ketoglutarate dehydrogenase gene of Escherichia coli k12. Journal of General Microbiology 71:207–220
    [Google Scholar]
  7. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. 1968; A proposal for a uniform nomenclature in bacterial genetics. Journal of General Microbiology 50:1–4
    [Google Scholar]
  8. Gholson R. K., Tritz G. J., Matney T. S., Andreoli A. J. 1969; Mode of nicotinamide adenine dinucleotide utilization by Escherichia coli . Journal of Bacteriology 99:895–896
    [Google Scholar]
  9. Guest J. R. 1974; Gene-protein relationships of the α-keto acid dehydrogenase complexes of Escherichia coli K12: chromosomal location of the lipoamide dehydrogenase gene. Journal of General Microbiology 80:523–532
    [Google Scholar]
  10. Guest J. R., Creaghan I. T. 1972; Lipoamide dehydrogenase mutants of Escherichia coli k12. Biochemical Journal 130:8p
    [Google Scholar]
  11. Guest J. R., Creaghan I. T. 1973; Gene-protein relationships of the a-keto acid dehydrogenase complexes of Escherichia coli K12: Isolation and characterization of lipoamide dehydrogenase mutants. Journal of General Microbiology 75:197–210
    [Google Scholar]
  12. Guest J. R., Creaghan I. T. 1974; Further studies with lipoamide dehydrogenase mutants of Escherichia coli kI2. Journal of General Microbiology 81:237–245
    [Google Scholar]
  13. Gunsalus I. C., Razzell W. E. 1957; Preparation and assay of lipoic acid and derivatives. In Methods in Enzymology 3 pp 941–946 Colowick S. P., Kaplan N. O. Edited by New York and London: Academic Press;
    [Google Scholar]
  14. Herbert A. A., Guest J. R. 1968; Biochemical and genetic studies with lysine + methionine mutants of Escherichia coli: lipoic and α-ketoglutarate dehydrogenase-less mutants. Journal of General Microbiology 53:363–381
    [Google Scholar]
  15. Herbert A. A., Guest J. R. 1969; Studies with α-ketoglutarate dehydrogenase mutants of Escherichia coli . Molecular and General Genetics 105:182–190
    [Google Scholar]
  16. Herbert A. A., Guest J. R. 1970; Two mutations affecting utilization of C4-dicarboxylic acids. Journal of General Microbiology 63:151–162
    [Google Scholar]
  17. Ikeda H., Tomizawa J. 1965; Transducing fragments in generalised transduction by phage P1. I. Molecular origin of the fragments. Journal of Molecular Biology 14:85–109
    [Google Scholar]
  18. Lennox E. S. 1955; Transduction of linked genetic characters of the host by bacteriophage Pi. Virology 1:190–206
    [Google Scholar]
  19. Lo T. C. Y., Rayman M. K., Sanwal B. D. 1972; Transport of succinate in Escherichia coli. I. Biochemical and genetic studies of transport in whole cells. Journal of Biological Chemistry 247:6323–6331
    [Google Scholar]
  20. Roth J. R. 1970; Genetic techniques in studies of bacterial metabolism. In Methods in Enzymology 17 A pp 3–35 Tabor H., Tabor C. W. Edited by New York and London: Academic Press;
    [Google Scholar]
  21. Sanderson K. E. 1970; Current linkage map of Salmonella typhimurium . Bacteriological Reviews 34:176–193
    [Google Scholar]
  22. Sanderson K. E. 1972; Linkage map of Salmonella typhimurium. Edition iv. Bacteriological Reviews 36:558–586
    [Google Scholar]
  23. Schmieger H. 1970; The molecular structure of the transducing particles of Salmonella-phage p22.II. Density gradient analysis of DNA. Molecular and General Genetics 109:323–337
    [Google Scholar]
  24. Schmieger H. 1972; Phage p22-mutants with increased or decreased transduction abilities. Molecular and General Genetics 119:75–88
    [Google Scholar]
  25. Shapiro J. A., Adhya S. L. 1969; The galactose operon of E. coli k-12. A deletion analysis of operon structure and polarity. Genetics 62:249–264
    [Google Scholar]
  26. Stouthamer A. H. 1969; A genetical and biochemical study of chlorate-resistant mutants of Salmonella typhimurium . Antonie van Leeuwenhoek 35:505–521
    [Google Scholar]
  27. Taylor A. L., Trotter C. D. 1972; Linkage map of Escherichia coli strain K-12. Bacteriological Reviews 36:504–524
    [Google Scholar]
  28. Vogel H., Bonner D. M. 1956; A convenient growth medium for Escherichia coli and some other microorganisms. Microbial Genetics Bulletin 13:43–44
    [Google Scholar]
  29. Wu T. T. 1966; A model for three-point analysis of random general transductions. Genetics 54:405–410
    [Google Scholar]
  30. Yura T., Wada C. 1968; Phenethyl alcohol resistance in Escherichia coli. I. Resistance of strain c6oo and its relation to azide resistance. Genetics 59:177–190
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-82-2-319
Loading
/content/journal/micro/10.1099/00221287-82-2-319
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error