1887

Abstract

SUMMARY: When and were grown in a chemostat, their morphology was related to growth rate. A transition from rod to coccus occurred at growth rates characteristic for each species; the higher the rate at which this transition occurred, the higher was the maximum specific growth rate. Organism size, concentration and viability were determined for at a range of dilution rates between 0·01 h and washout. Yield remained constant except at low dilution rates, although the number of organisms varied because of the changes in their size and shape. The percentage of viable organisms in the chemostat remained almost constant at dilution rates above 0·1 h (92 %) and was only slightly lowered even at dilution rates as low as 0·01 h.

Rods grown in the chemostat and then placed in nutrient-free solutions divided and produced cocci which survived for about 56 days. Cocci survived for about 70 days. The respiration rate of freshly harvested rods was higher than that of cocci but within two days both fell to low levels.

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/content/journal/micro/10.1099/00221287-82-2-213
1974-06-01
2024-12-09
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References

  1. Boylen C. W., Ensign J. C. 1970a; Long term starvation survival of rod and spherical cells of Arthro-bacter crystallopoietes. Journal of Bacteriology 103:569–577
    [Google Scholar]
  2. Boylen C. W., Ensign J. C. 1970b; Intracellular substrates for endogenous metabolism during longterm starvation of rod and spherical stage cells of Arthrobacter crystallopoietes. Journal of Bacteriology 103:578–587
    [Google Scholar]
  3. Boylen C. W., Pate J. L. 1973; Fine structure of Arthrobacter crystallopoietes during long term starvation of rod and spherical stage cells. Canadian Journal of Microbiology 19:1–5
    [Google Scholar]
  4. Gray T. R. G., Williams S. T. 1971; Microbial productivity in soil. In Microbes and Biological Productivity, Symposia of the Society for General Microbiology 21:256–286
    [Google Scholar]
  5. Herbert D. 1959; Some principles of continuous culture. In Recent Progress in Microbiology pp 381–396 Tunevall G. Edited by Oxford: Blackwell’s Scientific Publications;
    [Google Scholar]
  6. Jannasch H. W. 1969; Estimations of bacterial growth rates in natural water. Journal of Bacteriology 99:156–160
    [Google Scholar]
  7. Krulwich T. A., Ensign J. C. 1969; Alteration of glucose metabolism of Arthrobacter crystallopoietes by compounds which induce sphere-rod morphogenesis. Journal of Bacteriology 97:526–534
    [Google Scholar]
  8. Lowe W. E., Gray T. R. G. 1972; Ecological studies on coccoid bacteria in a pine forest soil. I. Classification. Soil Biology and Biochemistry 4:459–467
    [Google Scholar]
  9. Luscombe B. M., Gray T. R. G. 1971; Effect of varying growth rate on the morphology of Arthrobacter. Journal of General Microbiology 69:433–434
    [Google Scholar]
  10. Mulder E. G., Deinema M. H., Van Veen W. L., Zevenhuizen L.P.T.M. 1962; Polysaccharides, . lipids and poly-β-hydroxybutyrate in micro-organisms. Recueil des travaux chimiques des Pays-Bas et de la Belgique 81:797–809
    [Google Scholar]
  11. Owens J. D., Keddee R. M. 1969; The nitrogen nutrition of soil and herbage coryneform bacteria. Journal of Applied Bacteriology 33:338–347
    [Google Scholar]
  12. Pert S. J. 1972; Prospects and problems in continuous flow culture of micro-organisms. In Environmental Control of Cell Synthesis and Function pp 55–64 Dean A. C. R., Pirt S. J., Tempest D. W. Edited by London: Academic Press;
    [Google Scholar]
  13. Postgate J. R., Crumpton J. E., Hunter J. R. 1961; The measurement of bacterial viabilities by slide culture. Journal of General Microbiology 24:15–24
    [Google Scholar]
  14. Postgate J. R., Hunter J. R. 1962; The survival of starved bacteria. Journal of General Microbiology 39:233–263
    [Google Scholar]
  15. Powell E. O. 1956; Growth rate and generation time of bacteria with special reference to continuous culture. Journal of Microbiology 15:492–511
    [Google Scholar]
  16. Sober J. M., Charba J. F., Foust W. N. 1966; Endogenous metabolism of Azotobacter agilis. Journal of Bacteriology 93:687–695
    [Google Scholar]
  17. Tempest D. W. 1970; The continuous culture of micro-organisms: 1. Theory of the chemostat. In Methods in Microbiology 2 pp 259–276 Norris J. R., Ribbons D. W. Edited by London: Academic Press;
    [Google Scholar]
  18. Tempest D. W., Herbert D., Phipps P. J. 1967; Studies on the growth of Aerobacter aerogenes at low dilution rates in a chemostat. In Microbial Physiology and Continuous Culture pp 240–253 Powell E. O., Evans C. G. T., Strange R. E., Tempest D. W. Edited by London: H.M.S.O.;
    [Google Scholar]
  19. Veldkamp H. 1968; Bacterial physiology. In The Ecology of Soil Bacteria pp 201–219 Gray T. R. G., Parkinson D. Edited by Liverpool: Liverpool University Press;
    [Google Scholar]
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