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Volume 82, Issue 1

Yeast Killer Factor-induced Turbidity Changes in Cells and Sphaeroplasts of a Sensitive Strain Free

Abstract

Summary: When a culture of a sensitive yeast strain is treated with yeast killer-protein, there is an increase in turbidity. This killer protein-induced turbidity increase coincides with the loss of cellular ATP, and appears to be caused by increased light scattering owing to a reduction in volume of the treated yeast cells. Sphaeroplasts prepared from sensitive cells were also sensitive to killer protein, and a culture showed a large increase in turbidity when treated with killer protein. The increase in turbidity of sphaeroplasts was not accounted for by a change in volume, but did correlate with killer protein-induced alterations in membrane permeability, and is consistent with a killer protein-induced alteration in light scattering of the yeast cell membrane.

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© Society for General Microbiology 1974
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/content/journal/micro/10.1099/00221287-82-1-171
1974-05-01
2025-01-26
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/content/journal/micro/10.1099/00221287-82-1-171
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Yeast Killer Factor-induced Turbidity Changes in Cells and Sphaeroplasts of a Sensitive Strain
Microbiology 82, 171 (1974); https://doi.org/10.1099/00221287-82-1-171
/content/journal/micro/10.1099/00221287-82-1-171
/content/journal/micro/10.1099/00221287-82-1-171
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