1887

Abstract

SUMMARY Cytochromes of the and types have been detected in crude extracts of In the membrane fraction (P 144), cytochromes of the and types reduced by sulphide under anaerobic conditions were reoxidized by either oxygen or nitrite. Sulphite, however, reduced only cytochrome which was reoxidized by nitrate and oxygen but not by nitrite. In the S 144 fraction, cytochromes of the and types reduced by sulphite were reoxidized by oxygen but nitrate and nitrite were ineffective. Cytochromes of the and types combined with CO and these effects were reversed by light. Inhibitor studies on sulphide oxidation linked to either oxygen uptake or nitrite reduction indicated that flavin, a terminal oxidase and copper may be involved. A scheme of electron transport during sulphide and sulphite oxidation is proposed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-82-1-115
1974-05-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/82/1/mic-82-1-115.html?itemId=/content/journal/micro/10.1099/00221287-82-1-115&mimeType=html&fmt=ahah

References

  1. Adams C. A., Warnes G. M., Nicholas D. J. D. 1971; A sulphite-dependent nitrate reductase from Thiobacillus denitrificans . Biochimica et biophysica acta 235:398–406
    [Google Scholar]
  2. Aleem M.I.H. 1965; Thiosulphate oxidation and electron transport in Thiobacillus novellus . Journal of Bacteriology 90:95–101
    [Google Scholar]
  3. Aminuddin M., Nicholas D. J. D. 1973; Sulphide oxidation linked to the reduction of nitrate and nitrite in Thiobacillus denitrificans . Biochimica et biophysica acta 325:81–93
    [Google Scholar]
  4. Aminuddin M., Nicholas D. J. D. 1974; An AMP-independent sulphite oxidase from Thiobacillus denitrificans: purification and properties. Journal of General Microbiology 82:103–113
    [Google Scholar]
  5. Bowen T. J., Happold F. C., Taylor B. F. 1966; Studies on the adenosine-5ʹ-phosphosulphate from Thiobacillus denitrificans . Biochimica et biophysica acta 118:566–576
    [Google Scholar]
  6. Falk J. E. 1964 Porphyrins and Metalloproteins Amsterdam: Elsevier Publishing;
    [Google Scholar]
  7. Hewitt E. J., Nicholas D. J. D. 1968; Enzymes of inorganic nitrogen metabolism. In Modern Methods of Plant Analysis 7 pp 67–172 Linskens Y. F., Sanwal B. D., Tracey M. V. Edited by Berlin: Springer Verlag;
    [Google Scholar]
  8. Kodama A., Mori T. 1968; Studies on the metabolism of a sulphur oxidizing bacterium. V. Comparative studies of sulphur and sulphite oxidizing systems in Thiobacillus thio-oxidans . Plant and Cell Physiology 9:725–734
    [Google Scholar]
  9. Lam Y., Nicholas D. J. D. 1969; A nitrite reductase with cytochrome oxidase activity from Micrococcus denitrificans . Biochimica et biophysica acta 180:459–472
    [Google Scholar]
  10. Milhaud G., Aubert J. P., Millet J. 1958; Role physiologique due cytochrome c de la bacterie chimio autotrophe. Compte rendu hebdomadaire des séances de l’Academie des sciences 246:1766–1769
    [Google Scholar]
  11. Moriarty D. J. W., Nicholas D. J. D. 1969; Enzymic sulphide oxidation by Thiobacillus concretivorus . Biochimica et biophysica acta 184:114–123
    [Google Scholar]
  12. Moriarty D. J. W., Nicholas D. J. D. 1970; Electron transfer during sulphide and sulphite oxidation by Thiobacillus concretivorus . Biochemica et biophysica acta 216:130–138
    [Google Scholar]
  13. Newton N. 1969; The two haem nitrite reductase of Micrococcus denitrificans . Biochimica et biophysica acta 185:316–331
    [Google Scholar]
  14. Peck H. D. 1961; Evidence for the reversibility of the reaction catalysed by APS reductase. Biochimica et biophysica acta 49:621–624
    [Google Scholar]
  15. Peeters J., Aleem M. I. H. 1970; Oxidation of sulphur compounds and electron transport in Thiobacillus denitrificans . Archiv für Mikrobiologie 71:319–330
    [Google Scholar]
  16. Radcliffe B. C., Nicholas D. J. D. 1968; Some properties of a nitrite reductase from Pseudomonas denitrificans . Biochimica et biophysica acta 153:545–554
    [Google Scholar]
  17. Roy A. B., Trudinger P. A. 1970 The Biochemistry of Inorganic Compounds of Sulphur Cambridge University Press;
    [Google Scholar]
  18. Saxena J., Aleem M. I. H. 1973; Oxidation of sulphur compounds and coupled phosphorylation in the chemoautotroph, Thiobacillus neapolitanus . Canadian Journal of Biochemistry 51:560–568
    [Google Scholar]
  19. Trudinger P. A. 1961; Thiosulphate oxidation and cytochromes in Thiobacillus X. I. Fractionation of bacterial extracts and properties of cytochromes. Biochemical Journal 78:673–680
    [Google Scholar]
  20. Trudinger P. A. 1967; The metabolism of inorganic sulphur compounds by thiobacilli. Review of Pure and Applied Chemistry 17:1–24
    [Google Scholar]
  21. Yamanaka T. 1964; Identity of Pseudomonas cytochrome oxidase with Pseudomonas nitrite reductase. Nature; London: 204253–255
    [Google Scholar]
  22. Yamanaka T., Okunuki K. 1963; Crystalline Pseudomonas cytochrome oxidase. I. Enzymic properties with special reference to the biological specificity. Biochimica et biophysica acta 67:379–393
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-82-1-115
Loading
/content/journal/micro/10.1099/00221287-82-1-115
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error