1887

Abstract

SUMMARY: Fifteen strains of were agglutinated by 5 to 50 ng dextran/ml, and one by 500 ng/ml. Competitive inhibition of the agglutination by sugars or sugar alcohols suggested that the spatial arrangement of the HOC(3)H and O(1) groups were important in the dextran but that the dextran receptor is not identical with the dextran reactive site of glucosyl transferase. Periodate oxidation of the dextran, with or without subsequent borohydride reduction, prevented agglutination, again implicating the C3 group. Pretreatment of dextran with cyclohexyl-isocyanide or of with 4·0 -urea, 0·01 -EDTA or 0·1 % sodium dodecyl sulphate prevented agglutination; the last three reagents dispersed organisms already agglutinated. Ca or Mg reversed the effect of EDTA. Exposure of bacteria to 60 °C or to papain for a few minutes impaired agglutination, so the dextran receptor site may be protein.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-81-2-485
1974-04-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/81/2/mic-81-2-485.html?itemId=/content/journal/micro/10.1099/00221287-81-2-485&mimeType=html&fmt=ahah

References

  1. Akher M., Hamilton J. K., Montgomery R., Smith F. 1952; A new procedure for the determination of the fine structure of polysaccharides. Journal of the American Chemical Society 74:4970–4971
    [Google Scholar]
  2. Gibbons R. J., Fitzgerald R. J. 1969; Dextran-induced agglutination of Streptococcus mutans, and its potential role in the formation of microbial dental plaques. Journal of Bacteriology 98:341–346
    [Google Scholar]
  3. Gibbons R. J., Keyes P. H. 1969; Inhibition of insoluble dextran synthesis, plaque formation and dental caries in hamsters by low molecular weight dextran. Archives of Oral Biology 14:721–724
    [Google Scholar]
  4. Gibbons R.J, Nygaard M. 1968; Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci. Archives of Oral Biology 13:1249–1262
    [Google Scholar]
  5. Guggenheim B., Newbrun E. 1969; Extracellular glucosyltransferase activity of an HS strain of Streptococcus mutans . Helvetica odontologica acta 13:84–97
    [Google Scholar]
  6. Guggenheim B., Regolatt B., Mühlemann H. R. 1972; Caries and plaque inhibition by mutanase in rats. Caries Research 6:289–297
    [Google Scholar]
  7. Kelstrup J., Funder-Nielsen T. D. 1972; Molecular interactions between the extracellular polysaccharides of Streptococcus mutans . Archives of Oral Biology 17:1659–1670
    [Google Scholar]
  8. Kelstrup J., Funder-Nielsen T. D. 1973; Enzymatic reduction of the colonization of Streptococcus mutans in human dental plaque. Acta odontologica scandinavica 31:249–253
    [Google Scholar]
  9. Krasse B. 1966; Human streptococci and experimental caries in hamsters. Archives of Oral Biology 11:429–436
    [Google Scholar]
  10. Larm O., Lindberg B., Svensson S. 1971; Studies on the length of the side chains of the dextran elaborated by Leuconostoc mesenteroides . Carbohydrate Research 20:39–48
    [Google Scholar]
  11. Lindberg B., Svensson S. 1968; Structural studies of dextran from Leuconostoc mesenteroides nrrlb-512. Acta chemica scandinavica 22:1907–1912
    [Google Scholar]
  12. Rees D. A., Scott W. E. 1971; Polysaccharide conformation. VI. Journal of the Chemical Society B469–479
    [Google Scholar]
  13. de Stoppelaar J. D., König K. G., Plasschaert A. J. M., van der Hoeven J. S. 1971; Decreased cariogenicity of a mutant of Streptococcus mutans . Archives of Oral Biology 16:971–975
    [Google Scholar]
  14. Sundaralingam M. 1972; Molecular structures and conformations of the phospholipids and sphingomyelins. Annals of the New York Academy of Sciences 195:324–355
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-81-2-485
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error