1887

Abstract

SUMMARY

During bacteriophage studies of group A streptococci, some strains produced an inhibitory substance active against other group A strains. The inhibitory material was not found in broth cultures and could only be extracted from cultures grown in semi-solid agar; its activity was assayed by the inhibition of growth and killing of a sensitive group A strain, 12203. Viable counts showed that 2 h exposure to the factor at 37 °C produced greater than 90% inhibition and more than 60% killing of the treated culture. Turbidimetric measurements indicated no gross lysis of the sensitive culture and a dose-response curve demonstrated that approximately two bactericidal factor molecules were sufficient to inactivate a colony-forming unit of strain 12203. The inhibitory factor was of low molecular weight as judged by its diffusibility; it was concentrated by lyophilization and partially purified by gel filtration on G-10 Sephadex. The factor was resistant to proteolytic enzymes, nucleases and lipase as well as reducing and oxidizing conditions. Activity was lost at 60 °C, but the factor was stable at −70 °C. The bactericidal substance was stable within the pH range 2 to 12. These properties, as well as the wide range of activity of the bactericidal factor on several Gram-positive and -negative organisms, suggest that it is an inhibitory substance other than a bacteriocin.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-81-2-413
1974-04-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/81/2/mic-81-2-413.html?itemId=/content/journal/micro/10.1099/00221287-81-2-413&mimeType=html&fmt=ahah

References

  1. Bottone E., Allerhand J., Pisano M. A. 1971; Characteristics of a bacteriocin derived from Streptococcus faecalis var. zymogenes antagonistic to Diplococcus pneumoniae. Applied Microbiology 22:200–204
    [Google Scholar]
  2. Brock T. D., Preacher B., Pierson D. 1963; Survey of the bacteriocines of enterococci. Journal of Bacteriology 86:702–707
    [Google Scholar]
  3. Dochez A. R., Avery O. T., Lancefield R. C. 1919; Studies on the biology of streptococcus. I. Antigenic relationships between strains of Streptococcus haemolyticus. Journal of Experimental Medicine 30:179–213
    [Google Scholar]
  4. Gale E. F., Gundliffe E., Reynolds P. E., Richmond M. H., Waring M. J. 1972 The Molecular Basis of Antibiotic Action New York: John Wiley;
    [Google Scholar]
  5. Hurst A. 1966; Biosynthesis of the antibiotic nisin and other basic peptides by Streptococcus lactis grown in batch culture. Journal of General Microbiology 45:503–513
    [Google Scholar]
  6. Kelstrup J., Gibbons R. J. 1969; Bacteriocins from human and rodent streptococci. Archives of Oral Biology 14:251–258
    [Google Scholar]
  7. Kramer J., Brandis H. 1972; Charakterisierung eines Streptococcus agalactiae-Bacteriocins. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I) 219:290–301
    [Google Scholar]
  8. Kuttner A. G. 1966; Production of group A streptococcal bacteriocines with special reference to nephritogenic types. Journal of Experimental Medicine 124:279–291
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Mattick A. T. R., Hirsch A. 1947; Further observations on an inhibitory substance (nisin) from lactic streptococci. Lancet 2:5–8
    [Google Scholar]
  11. Maxted W. R., Potter E. V. 1967; The presence of type 12 m-protein antigen in group G streptococci. Journal of General Microbiology 49:119–125
    [Google Scholar]
  12. Overture G. D., Mortimer E. A. 1970; Studies of the relationship between the production of bacteriocines by group A streptococci and acute glomerulonephritis. Journal of Experimental Medicine 132:694–701
    [Google Scholar]
  13. Oxford A. E. 1944; Diplococcin, an antibacterial protein elaborated by certain milk streptococci. Journal of Biochemistry 38:178–182
    [Google Scholar]
  14. Reeves P. 1972 The Bacteriocins New York: Springer-Verlag;
    [Google Scholar]
  15. Schlegel R., Slade H. D. 1972; Bacteriocin production by transformable group H streptococci. Journal of Bacteriology 112:824–829
    [Google Scholar]
  16. Schlegel R., Slade H. D. 1973; Properties of a Streptococcus sanquis (group H) bacteriocin and its separation from the competence factor of transformation. Journal of Bacteriology 115:655–661
    [Google Scholar]
  17. Sherwood N. P., Russel B. E., Jay A. R., Bowman K. 1949; Studies on streptococci. III. New antibiotic substances produced by beta hemolytic streptococci. Journal of Infectious Disease 84:88–91
    [Google Scholar]
  18. Zabriskie J. B. 1964; The role of temperate bacteriophage in the production of erythrogenic toxin by group A streptococci. Journal of Experimental Medicine 119:761–779
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-81-2-413
Loading
/content/journal/micro/10.1099/00221287-81-2-413
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error