1887

Abstract

The NADP-specific glutamate dehydrogenase (NADP-GDH) activity of was rapidly lost from cultures starved for a carbon source. This loss of NADP-GDH was blocked by protein synthesis inhibitors. Glutamate repressed NADP-GDH but did not cause rapid loss of activity. Since NADP-GDH is involved in the participation of ammonium in the regulation of nitrogen metabolism, the loss of NADP-GDH activity accompanying carbon starvation may be important in the interaction between carbon and nitrogen metabolism. Increased NAD specific glutamate dehydrogenase activity (NAD-GDH) was observed when mycelium was transferred to medium lacking glucose. The increase in NAD-GDH activity was greatest when glutamate was present. Protein synthesis inhibitors did not prevent this increase in activity.

Two mutants, 102 and 19, which are altered in regulation of nitrogen metabolism, are similar to the wild-type strain with regard to regulation of NADP-GDH and NAD-GDH.

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/content/journal/micro/10.1099/00221287-81-1-165
1974-03-01
2022-01-29
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References

  1. Arst H. N., Cove D. J. 1973; Nitrogen metabolite repression in Aspergillus nidulans. Molecular and General Genetics in the Press
    [Google Scholar]
  2. Arst H. N., MacDonald D. W. 1973; A mutant of Aspergillus nidulans lacking NADP-linked glutamate dehydrogenase. Molecular and General Genetics 122:261–266
    [Google Scholar]
  3. Dubois E., Grenson M., Wiame J. M. 1973; Release of the ‘ammonia effect’ on three catabolic enzymes of NADP-specific glutamate dehydrogenaseless mutations in Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications 50:967–972
    [Google Scholar]
  4. Ferguson J. J., Boll M., Holzer H. 1967; Yeast malate dehydrogenase: enzyme inactivation in catabolite repression. European Journal of Biochemistry 1:21–25
    [Google Scholar]
  5. Ferguson A. R., Sims A. P. 1971; Inactivation in vivo of glutamine synthetase and NAD-specific glutamate dehydrogenase: its role in the regulation of glutamine synthesis in yeasts. Journal of General Microbiology 69:423–427
    [Google Scholar]
  6. Hynes M. J. 1970; Induction and repression of amidase enzymes in Aspergillus nidulans. Journal of Bacteriology 103:482–487
    [Google Scholar]
  7. Hynes M. J. 1972; Mutants with altered glucose repression of amidase enzymes in Aspergillus nidulans. Journal of Bacteriology 111:717–722
    [Google Scholar]
  8. Hynes M. J. 1973a; Pleiotropic mutants affecting control of nitrogen metabolism in Aspergillus nidulans. Molecular and General Genetics 125:99–107
    [Google Scholar]
  9. Hynes M. J. 1973b; Alterations in the control of glutamate uptake in mutants of Aspergillus nidulans. Biochemical and Biophysical Research Communications 54:685–689
    [Google Scholar]
  10. Hynes M. J. 1973c; The effect of lack of a carbon source on nitrate-reductase activity in Aspergillus nidulans. Journal of General Microbiology 79:155–157
    [Google Scholar]
  11. Kapoor M., Grover A. K. 1970; Catabolite-controlled regulation of glutamate dehydrogenases of Neurospora crassa. Canadian Journal of Microbiology 16:33–40
    [Google Scholar]
  12. Katsunuma T., Schött E., Elsässer S., Holzer H. 1972; Purification and properties of tryptophan-synthase-inactivating enzymes from yeast. European Journal of Biochemistry 27:520–526
    [Google Scholar]
  13. Kinghorn J. R., Pateman J. A. 1973; The regulation of nicotinamide-adenine-dinucleotide glutamate dehydrogenase in Aspergillus nidulans. Biochemical Society Transactions 1:675–676
    [Google Scholar]
  14. Lewis C. M., Fincham J. R. S. 1970; Regulation of nitrate reductase in the basidiomycete Ustilago maydis. Journal of Bacteriology 103:55–61
    [Google Scholar]
  15. Pateman J. A. 1969; Regulation of synthesis of glutamate dehydrogenase and glutamine synthetase in microorganisms. Biochemical Journal 115:769–775
    [Google Scholar]
  16. Pateman J. A., Kinghorn J. R., Dunn E., Forbes E. 1973; Ammonium regulation in Aspergillus nidulans. Journal of Bacteriology 114:943–950
    [Google Scholar]
  17. Schimke R. T., Doyle D. 1970; Control of enzyme levels in animal tissues. Annual Review of Biochemistry 39:929–976
    [Google Scholar]
  18. Subramanian K. N., Sorger G. J. 1972; Regulation of nitrate reductase in Neurospora crassa: stability in vivo. Journal of Bacteriology no:538–546
    [Google Scholar]
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