@article{mbs:/content/journal/micro/10.1099/00221287-81-1-131, author = "Wallenfels, Barbara and Jann, K.", title = "The Action of Bacteriophage Ω8 on Two Strains of Escherichia coli 08", journal= "Microbiology", year = "1974", volume = "81", number = "1", pages = "131-144", doi = "https://doi.org/10.1099/00221287-81-1-131", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-81-1-131", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: Bacteriophage Ω 8 is propagated in Escherichia coli e56b (o8: K27−: H−), a non-capsulated strain. Another non-capsulated strain, E. coli 2398 (o8:K?−:H−), is killed by bacteriophage Ω 8 without phage propagation. This strain was formerly believed to be E. coli 093:K?−:H−, cross-reacting with strain e56b. We have established chemical and serological identity of the o8-specific lipopolysaccharides of the two strains. The o8-specific lipopolysaccharides of both strains inhibited the infection of Escherichia coli e56b with bacteriophage Ω 8 equally well. The adsorption rate constants of Ω 8 were identical for the two strains of E. coli o8. Evidence was obtained with 32P-labelled bacteriophage Ω 8 for penetration of viral DNA into both bacteria] strains. In host strain e56b, phage particle synthesis occurred normally. In strain 2398 the viral DNA was not degraded but its expression was blocked. The killing effect of D 8 on E. coli strain 2398 is supposed to be due to damage of the cytoplasmic membrane, which could not be reversed under the influence of viral information. This was indicated by a blockage of cellular respiration, β-galactoside transport and RNA as well as protein synthesis.", }