Two representative lipoamide dehydrogenase mutants ( and ) were used to locate the gene in the linkage map of by conjugation and detailed transductional analysis with phage P1. Time of entry mapping indicated that the gene was between and and very close to Average cotransduction frequencies between and other markers were: (97 %), (93 %), (84 %), (71 %), (64 %), (8 to 41 %), (38 %), (21 %), (17 %) and and gal (< 1 %). These values, together with the contransduction frequencies for many other pairs of markers in this region and the results of three-factor crosses, established the gene order The very close proximity of and suggested that they may be contiguous and that the gene may be the distal gene in the pyruvate dehydrogenase operon. In this case, the expression of the gene may be governed by a secondary transcriptional promoter in the region in order to generate lipoamide dehydrogenase components for assembly of the α-ketoglutarate dehydrogenase complex. Other possible mechanisms are also discussed.


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