1887

Abstract

SUMMARY

DNA-DNA reassociation was studied in order to determine the genomic relatedness among 26 species of whorl-forming streptomycetes. DNA from 5052, At-37 and At-267 which showed a low relationship with each other were selected as the reference samples. Relative reassociation values of various streptoverticillia compared with these reference DNAs ranged from 25 to 82% and most were less than 50%. These results suggest that the whorl-forming streptomycetes are a mixture of heterogeneous species when considered at the genomic level. The results also suggest close relationships between 5053 and 5259 and also among 5253, 5038 and var. At-79. The close relationships within the latter three species were confirmed by their DNA reassociation with reference DNA and by thermal dissociation profiles of the hybrid DNAs on membrane filters. From data obtained, the suitability of several diagnostic features as taxonomic criteria was discussed.

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/content/journal/micro/10.1099/00221287-80-2-507
1974-02-01
2022-01-22
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References

  1. Baldacci E. 1958; Development in the classification of Actinomycetes. Giornale di Microbiologia 6:10–27
    [Google Scholar]
  2. Brenner D. J., Falkow S. 1971; Molecular relationships among members of the Enterobacteriaceae. Advances in Genetics 16:81–118
    [Google Scholar]
  3. Gibbins A. M., Gregory K. F. 1972; Relatedness among Rhizobium and Agrobacterium species determined by three methods of nucleic acid hybridization. Journal of Bacteriology 111:129–141
    [Google Scholar]
  4. Hütter R. 1967 Systematik der Streptomyceten Basel: Karger;
    [Google Scholar]
  5. Locci R., Baldacci E., Baldan B. P. 1969; The genus Streptoverticillium. A taxonomic study. Giornale di Microbiologia 17:1–60
    [Google Scholar]
  6. Okanishi M., Akagawa H., Umezawa H. 1972; An evaluation of taxonomic criteria in streptomycetes on the basis of deoxyribonucleic acid homology. Journal of General Microbiology 72:49–58
    [Google Scholar]
  7. Okanishi M., Gregory K. F. 1970; Methods for the determination of deoxyribonucleic acid homo-logies in Streptomyces. Journal of Bacteriology 104:1086–1094
    [Google Scholar]
  8. Palleroni N. J., Ballard R. W., Ralston E., Doudoroff M. 1972; Deoxyribonucleic acid homologies among some Pseudomonas species. Journal of Bacteriology 110:1–11
    [Google Scholar]
  9. Pridham T. G., Hesseltine C. W., Benedict R. G. 1958; A guide for the classification of Streptomyces according to selected groups.Placement of strain in morphological section. Applied Microbiology 6:52–79
    [Google Scholar]
  10. Rogul M., Brendle J. J., Haapala D. K., Alexander A. D. 1970; Nucleic acid similarities among Pseudomonas pseudomallei, Pseudomonas multivorans, and Actinobacillus mallei. Journal of Bacteriology 101:827–835
    [Google Scholar]
  11. Sagara Y., Fukui K., Ota F., Yoshida N., Kashiyama T., Fujimoto M. 1971; Rapid formation of protoplasts of Streptomyces griseoflavus and their fine structure. Japanese Journal of Microbiology 15:73–84
    [Google Scholar]
  12. Sakagami Y., Yamaguchi I., Yonehara H., Okimoto Y. Y., Yamanouchi S., Takiguchi K., Sakai H. 1958; Latumcidin, a new antibiotic from Streptomyces sp. Journal of Antibiotics 11A:6–13
    [Google Scholar]
  13. Shinobu R. 1965; Taxonomy of the whorl-forming streptomycetaceae. Memoirs of the Osaka University of the Liberal Arts and Education B14:72–179
    [Google Scholar]
  14. Shirling E. B., Gottlieb D. 1968a; Cooperative description of type cultures of Streptomyces. II. Species description from first study. International Journal of Systematic Bacteriology 18:69–189
    [Google Scholar]
  15. Shirling E. B., Gottlieb D. 1968b; Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. International Journal of Systematic Bacteriology 18:279–392
    [Google Scholar]
  16. Ulitzur S. 1972; Rapid determination of DNA base composition by ultraviolet spectroscopy. Biochimica et biophysica acta 272:1–11
    [Google Scholar]
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