The localization of total and newly synthesized [H]prenol in was investigated by autoradiography of electron micrographs of bacteria grown with [H]mevalonic acid. The distribution of radioactive grains differed from a random distribution in being concentrated in the membrane; this confirmed earlier chemical studies. The distribution of total [H]prenol along the cell, after continuous growth with [H]mevalonic acid, did not indicate any specific localization. Newly synthesized prenol, located after a 20 min pulse with [H]mevalonic acid, was also uniformly distributed except in organisms which had spent the previous 20 min forming septa. In these bacteria there was a concentration of prenol in the septal region.


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