1887

Microbiology Society logo

Volume 80, Issue 2

The Release of Potassium Ions from Candida albicans in the Presence of Polyene Antibiotics Free

Abstract

SUMMARY: The addition of amphotericin methyl ester (AME) to suspensions of is followed by a progressively increasing rate of release of K from within the cell. The time taken for a given rate of release to be reached depends upon the AME concentration (the product of AME concentration and time being approximately constant), the pH and concentration of buffer, the temperature, the suspension density, and the phase of growth of the organisms. The sensitivity of to AME decreases during the growth of a batch culture, rapid decrease occurring after the organisms have reached the stationary phase of growth. The induction of K release does not occur at 0 °C and shows a high temperature coefficient over the range 15 to 30 °C.

Suspensions of mouse LS cells are 10 to 30 times less sensitive than towards AME, amphotericin B and nystatin; the two types of cell display little difference in sensitivity towards perimycin and candicidin, while mouse LS cells are more sensitive than towards filipin.

Addition of sterols at the same time as AME increases the time taken for the release of K to reach a given rate; of the sterols tested, zymosterol was the most effective in antagonizing AME, while ergosterol was approximately twice as effective as cholesterol on a molar basis. When organisms were exposed to AME for a short time, removed and resuspended in the absence of drug, the rate of Krelease continued to increase; this increase was prevented by ergosterol: 2 × 10 -ergosterol prevented further damage after organisms had been exposed to 10 AME.

  • Received:
  • Revised:
  • Published Online:
© Society for General Microbiology 1974
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-80-2-451
1974-02-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/80/2/mic-80-2-451.html?itemId=/content/journal/micro/10.1099/00221287-80-2-451&mimeType=html&fmt=ahah

References

  1. Andreoli T. E., Monahan M. 1968; The interaction of polyene antibiotics with thin lipid membranes. Journal of General Physiology 52:300–325
     [Google Scholar]
  2. Bittman R., Fischkoff S. A. 1972; Fluorescence studies of the binding of the polyene antibiotics filipin III, amphotericin B, nystatin and lagosin to cholesterol. Proceedings of the National Academy of Sciences of the United States of America 69:3795–3799
     [Google Scholar]
  3. Borowski E., Cybulska B. 1967; Potassiumless death of Saccharomyces cerevisiae cells treated with N-succinyl perimycin and the reversal of fungicidal action of the antibiotic by potassium ions. Nature; London: 2131034–1035
     [Google Scholar]
  4. Cass A., Finkelstein A., Krespi V. 1970; The ion permeability induced in thin lipid membranes by the polyene antibiotics nystatin and amphotericin B. Journal of General Physiology 56:100–124
     [Google Scholar]
  5. Eagle H. 1959; Amino acid metabolism in mammalian cell cultures. Science; New York: 130432–437
     [Google Scholar]
  6. Finkelstein A., Cass A. 1968; Permeability and electrical properties of thin lipid membranes. Journal of General Physiology 52:145–173
     [Google Scholar]
  7. Garcia-Mendoza C., Villanueva J. R. 1962; Production of yeast protoplasts by an enzyme preparation of Streptomyces sp. Nature; London: 1951326–1327
     [Google Scholar]
  8. Gottlieb D., Carter H. E., Wu L. C., Sloneker J. H. 1960; Inhibition of fungi by filipin and its antagonism by sterols. Phytopathology 50:594–603
     [Google Scholar]
  9. Holts R., Finkelstein A. 1970; The water and non-electrolyte permeability induced in thin lipid membranes by the polyene antibiotics, nystatin and amphotericin B. Journal of General Physiology 56:125–145
     [Google Scholar]
  10. Lampen J. O. 1966; Interference by polyene antifungal antibiotics (especially nystatin and filipin) with special membrane functions. Symposia of the Society for General Microbiology 16:111–130
     [Google Scholar]
  11. Lampen J. O., Arnow P. M., Borowska Z., Laskin A. I. 1962; Location and role of sterol at nystatin binding sites. Journal of Bacteriology 84:1152–1160
     [Google Scholar]
  12. Lampen J. O., Gill J. W., Arnow P. M., Magana-Plaza L. 1963; Inhibition of the pleuropneumonialike organism Mycoplasma gallisepticum by certain polyene antifungal antibiotics. Journal of Bacteriology 86:945–949
     [Google Scholar]
  13. Mechlinski W., Schaffner C. P. 1972; Polyene macrolide derivatives. I. N-acylation and esterification reactions with amphotericin B. Journal of Antibiotics 25:256–258
     [Google Scholar]
  14. Mechlinski W., Schaffner C. P., Ganis P., Avitabile G. 1970; Structure and absolute configuration of the polyene macrolide antibiotic amphotericin B. Tetrahedron Letters 44:3873–3876
     [Google Scholar]
  15. Norman A. W., Demel R. A., de Kruyff B., Guertsvankessel W. S. H., van Deenen L. L. M. 1972; Studies on the biological properties of polyene antibiotics: comparison of other antibiotics with filipin in their ability to interact specifically with sterol. Biochimica et biophysica acta 290:1–14
     [Google Scholar]
  16. Schaffner C. P. 1973; The biochemical implications of polyene macrolide-sterol interaction. Proceedings of the International Fermentation Symposium in the Press
    [Google Scholar]
  17. Verkleij A. J., de Kruijff B., Gerritsen W. G., Demel R. A., van Deenen L. L. M., Ververgaert P. H. J. 1973; Freeze-etch electron microscopy of erythrocytes, Acholeplasma laidlawii cells and liposomal membranes after the action of filipin and amphotericin B. Biochimica et biophysica acta 291:577–581
     [Google Scholar]
  18. Weber M. M., Kinsky S. C. 1965; Effect of cholesterol on the sensitivity of Mycoplasma laidlawii to the polyene antibiotic filipin. Journal of Bacteriology 89:306–312
     [Google Scholar]
  19. Zygmunt W. A. 1966; Intracellular loss of potassium in Candida albicans after exposure to polyene antifungal antibiotics. Applied Microbiology 14:953–956
     [Google Scholar]
  20. Zygmunt W. A., Tavormina P. A. 1966; Steroid interference with antifungal activity of polyene antibiotics. Applied Microbiology 14:865–869
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-80-2-451
Loading
The Release of Potassium Ions from Candida albicans in the Presence of Polyene Antibiotics
Microbiology 80, 451 (1974); https://doi.org/10.1099/00221287-80-2-451
/content/journal/micro/10.1099/00221287-80-2-451
/content/journal/micro/10.1099/00221287-80-2-451
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error