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During a 17-day period the mycoflora of the barley phyllosphere was assessed by microscopic observation of leaf impressions. The frequency of fungal colonies detected was 0·1 to 17·2 per cm2 of leaf. In favourable conditions the leaf area occupied by each colony was about 0·05 mm2. The total surface of colonization could thus reach 2 mm2/cm2, but generally varied between 0·002 and 0·774 mm2. Only a limited range of species was observed, predominantly Cladosporium spp. The highest active populations coincided with a period of massive emission of pollen.
The quantitative results obtained bore little relation to those from leaf-washing counts: the reasons for this are discussed.
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