@article{mbs:/content/journal/micro/10.1099/00221287-80-1-51, author = "Satta, G. and Fontana, Roberta", title = "Characterization of a Conditional Mutant with Altered Envelope Showing pH-dependent Morphology and Temperature-dependent Division", journal= "Microbiology", year = "1974", volume = "80", number = "1", pages = "51-63", doi = "https://doi.org/10.1099/00221287-80-1-51", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-80-1-51", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: The properties of a conditional mutant of Klebsiella pneumoniae (Mir M7) are described. The mutant has a pH-dependent disturbance of morphology and a temperature-dependent disturbance of division. At pH 7 the mutant grows as cocci and at 41 °C (restrictive temperature) division is inhibited. At acid pH and restrictive temperature, filaments are formed. At pH 7 and permissive temperature, polymorphous cocci are obtained. At pH 7 and 41 °C, giant polymorphous cells are formed. The inhibition of division is phenotypically reverted by 0·05 m-Mg2+, 0·1 m-Na+ or 0·4 m-sucrose. Seventeen revertants were isolated having exactly the same characteristics as the wild-type Klebsiella, so it seems that only one mutation is responsible for the different disturbances of the mutant. The possibility of membrane damage on which the morphology and division disturbances may depend is discussed.", }