1887

Abstract

SUMMARY: 86 organisms with impaired walls were prepared by three methods and the effect of antibacterial agents on their growth studied. The growth of penicillin spheroplasts was masked by the overgrowth of unaltered cells in the culture; the EDTA-lysozyme spheroplasts themselves were non-viable. The growth of penicillin spheroplasts was not affected by cell-wall inhibitors and ampicillin suppressed the overgrowth of unaltered cells. The sensitivity of penicillin spheroplasts and parent cells to inhibition by a range of agents was similar. EDTA treatment enhanced the susceptibility of 86 and other strains of Gramnegative species to several antibiotics, particularly erythromycin. Polyacetic acid chelating agents related to EDTA and some new amides derived from glycine, alanine, phenylalanine or methionine also potentiated erythromycin Erythromycin showed some activity in protecting mice against infection by EDTA- treated 86. The antibiotic did not protect against infection by the untreated bacteria and its activity was not greatly enhanced by simultaneous administration of EDTA or the amide derivatives.

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1974-01-01
2021-08-03
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References

  1. Albert A. 1968; Stability constants of some complexing agents. In Selective Toxicity p. 318 London: Methuen;
    [Google Scholar]
  2. Gauze G. G., Loshkaseva N. P., Yu Y. D. 1965; Mechanism of oligomycin action. Federal Proceedings Translation Supplement 25:T.329–T.332
    [Google Scholar]
  3. Gilby A. R., Few A. V. 1960a; Lysis of protoplasts of Micrococcus lysodeikticus by ionic detergents. Journal of General Microbiology 23:19–26
    [Google Scholar]
  4. Gilby A. R., Few A. V. 1960b; Lysis of protoplasts of Micrococcus lysodeikticus by alcohol. Journal of General Microbiology 23:27–33
    [Google Scholar]
  5. Glauert A. M., Thornley M. J. 1966; Glutaraldehyde fixation of Gram-negative bacteria. Journal of the Royal Microscopical Society 85:449–453
    [Google Scholar]
  6. Glauert A. M., Thornley M. J. 1969; Topography of the bacterial cell wall. Annual Review of Microbiology 23:159–198
    [Google Scholar]
  7. Goldschmidt M. C., Wyss O. 1966; Chelation effects on Azotobacter cells and cysts. Journal of Bacteriology 91:120–124
    [Google Scholar]
  8. Goldschmidt M. C., Wyss O. 1967; The role of tris in EDTA toxicity and lysozyme lysis. Journal of General Microbiology 47:421–431
    [Google Scholar]
  9. Hamilton W. A. 1968; The mechanism of the bacteriostatic action of tetrachlorosalicylanilide: a membrane- active antibacterial compound. Journal of General Microbiology 50:441–458
    [Google Scholar]
  10. Hamilton-Miller J. M. T. 1966; Damaging effects of EDTA and penicillins on permeability barriers in Gram-negative bacteria. Biochemical Journal 100:675–682
    [Google Scholar]
  11. Hancock R., Fitz-James P. C. 1964; Some differences in the action of penicillin, bacitracin and vancomycin on Bacillus megaterium . Journal of Bacteriology 87:1044–1050
    [Google Scholar]
  12. Haywood A. M., Sinsheimer R. L. 1963; Inhibition of protein synthesis in Escherichia coli protoplasts by actinomycin D. Journal of Molecular Biology 6:247–249
    [Google Scholar]
  13. Hirokawa H. 1962; Biochemical and cytological observations during the reversing process from spheroplasts to rod form cells in Escherichia coli . Journal of Bacteriology 84:1161–1168
    [Google Scholar]
  14. Hirokawa H. 1966; Properties of rod cells reversed from penicillin spheroplasts in Escherichia coli . Journal of Bacteriology 91:125–128
    [Google Scholar]
  15. Hugo W. B., Russell A. D. 1960; Quantitative aspects of penicillin action on Escherichia coli in hypertonic media. Journal of Bacteriology 80:436–440
    [Google Scholar]
  16. Kay D., Fildes P. 1950; The calcium requirement of a typhoid bacteriophage. British Journal of Experimental Pathology 31:338–348
    [Google Scholar]
  17. Leive L. 1965a; Actinomycin sensitivity in Escherichia coli produced by EDTA. Biochemical and Biophysical Research Communications 18:13–17
    [Google Scholar]
  18. Leive L. 1965b; A non-specific increase in permeability in Escherichia coli produced by EDTA. Proceedings of the National Academy of Sciences of the United States of America 53:745–750
    [Google Scholar]
  19. Leive L. 1965c; Release of lipopolysaccharide by EDTA treatment of Escherichia coli . Biochemical and Biophysical Research Communications 21:290–296
    [Google Scholar]
  20. Leive L. 1968; Studies on the permeability change produced in coliform bacteria by EDTA. Journal of Biological Chemistry 243:2373–2380
    [Google Scholar]
  21. Litchfield J. T. 1949; A method for rapid graphic solution of time per cent effect curves. Journal oj Pharmacology and Experimental Therapeutics 97:399–408
    [Google Scholar]
  22. Loveless L. E., Spoerl E., Weisman T. H. 1954; A survey of effects of chemicals on division and growth of yeast and Escherichia coli . Journal of Bacteriology 68:637–644
    [Google Scholar]
  23. Mach B., Tatum E. L. 1963; Ribonucleic acid synthesis in protoplasts of Escherichia coli. Inhibition by actinomycin D. Science; New York: 1391051–1052
    [Google Scholar]
  24. Mao J. C.-H., Putterman M. 1968; Accumulation in Gram-positive and Gram-negative bacteria as a mechanism of resistance to erythromycin. Journal of Bacteriology 95:1111–1117
    [Google Scholar]
  25. Muschel L. H., Gustafson L. 1968; Antibiotic, detergent, and complement sensitivity of Salmonella typhi after ethylene-diaminetetraacetic acid treatment. Journal of Bacteriology 95:2010–2013
    [Google Scholar]
  26. Neu H. C. 1969; The role of amine buffers in EDTA toxicity and their effect on osmotic shock. Journal of General Microbiology 57:215–220
    [Google Scholar]
  27. Razin S., Argaman M. 1963; Lysis of Mycoplasma, bacterial protoplasts, spheroplasts and L-forms by various agents. Journal of General Microbiology 30:155–172
    [Google Scholar]
  28. Reich E. 1966; Binding to DNA and inhibition of DNA functions by actinomycins. In Biochemical Studies of Antimicrobial Drugs. XVIth Symposium of the Society for General Microbiology pp. 266–280 Cambridge University Press;
    [Google Scholar]
  29. Reynolds P. E. 1966; Antibiotics affecting cell-wall synthesis. Biochemical Studies of Antimicrobial Drugs. XVIth Symposium of the Society for General Microbiology pp. 47–69
    [Google Scholar]
  30. Schnaitman C. A. 1971; The effect of ethylene-diaminetetraacetic acid, Triton X-100, and lysozyme on the morphology and chemical composition of isolated cell walls of Escherichia coli . Journal of Bacteriology 108:553–563
    [Google Scholar]
  31. Shockman G. D., Lampen J. O. 1962; Inhibition by antibiotics of the growth of bacterial and yeast protoplasts. Journal of Bacteriology 84:508–512
    [Google Scholar]
  32. Vazquez D. 1966; Mode of action of chloramphenicol and related antibiotics. In Biochemical Studies of Antimicrobial Drugs. XVIth Symposium of the Society for General Microbiology pp. 169–191 Cambridge University Press;
    [Google Scholar]
  33. Voss J. G. 1964; Lysozyme lysis of Gram-negative bacteria without production of spheroplasts. Journal of General Microbiology 35:313–317
    [Google Scholar]
  34. Voss J. G. 1967; Effect of organic cations on the Gram-negative cell wall and their bactericidal activity with EDTA and surface-active agents. Journal of General Microbiology 48:391–400
    [Google Scholar]
  35. Weiser R., Asscher A. W., Wimpenny J. 1968; In vitro reversal of antibiotic resistance by ethylenedia- mine tetraacetic acid. Nature; London: 2191365–1366
    [Google Scholar]
  36. West T. S. 1969 Complexometry with EDTA and Related Reagents p. 223 B.D.H. Chemicals Ltd;
    [Google Scholar]
  37. Work E., Knox K. W., Vesk M. 1966; The chemistry and electron microscopy of an extracellular lipopolysaccharide from Escherichia coli . Annals of the New York Academy of Sciences 133:438–449
    [Google Scholar]
  38. Yudkin M. D. 1963; The effect of penicillin, novobiocin, streptomycin and vancomycin on membrane synthesis by protoplasts of Bacillus megaterium . Biochemical Journal 89:290–296
    [Google Scholar]
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