SUMMARY: Considerable amounts of aflatoxins were produced when mycelium of NRRL3240 grown for 4 days in a synthetic medium (SL medium) or a semi-synthetic medium (YES medium) were resuspended in either medium for 2 days, but not when resuspended in media lacking sucrose, or in buffers. Acetate[I-C] was incorporated efficiently on addition to cultures grown in YES medium or on addition to mycelium grown and resuspended in SL medium. Changes in pH within the range of 3.5 to 6.5 did not have any pronounced effect on aflatoxin production or on incorporation of labelled acetate by resuspended mycelia.


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