SUMMARY: Studies with whole cells and crude cell-free extracts of A3(2) indicate that its RNA polymerase, though sensitive to rifampicin, is less so than most bacterial RNA polymerases. Of two other streptomycetes examined, one resembled A3(2) and the other was more like other prokaryotes in respect of rifampicin sensitivity of its RNA polymerase. Three genetic map locations for rifampicin-resistance () mutations of A3(2) were determined, one () at 6 o'clock on the conventional linkage map, very close to , one () at 11 o'clock, between and , and a third () at 7 o'clock, between and In representative and mutants, RNA synthesis was resistant to rifampicin in whole cells, but not in extracts, whereas in several mutants RNA synthesis was equally resistant both in whole cells and in extracts, suggesting that the gene may specify part of the RNA polymerase. None of more than 100 mutants isolated possessed morphological abnormalities attributable to the mutation.


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