Studies on the Pyocins of Production of Contractile and Flexuous Pyocins in Free

Abstract

SUMMARY

Strains of () were examined for pyocin production by means of a standard pyocin-typing technique and also a modified method which depends on the ability of some pyocins to pass through a cellulose acetate membrane. The results suggested that strains could produce (1) pyocins incapable of spontaneous passage through the membrane, (2) pyocins capable of passage through the membrane, (3) both kinds of pyocin simultaneously. Electron microscopy revealed that pyocins in the first circumstance included contractile pyocins and those in the second included flexuous, rod-like particles. 430 produced both contractile and flexuous pyocins simultaneously. The pyocins could be separated by absorption with sensitive bacteria or by gel filtration. The two pyocins differed serologically and in their range of inhibitory activity against strains of .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-80-1-17
1974-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/80/1/mic-80-1-17.html?itemId=/content/journal/micro/10.1099/00221287-80-1-17&mimeType=html&fmt=ahah

References

  1. Amako K., Yasunaka K., Takeya K. 1970; Relationship between rhapidosome and pyocin in Pseudomonas fluorescens. Journal of General Microbiology 62:107–112
    [Google Scholar]
  2. Bradley D. E. 1966; The structure and infective process of a Pseudomonas aeruginosa bacteriophage containing ribonucleic acid. Journal of General Microbiology 45:83–96
    [Google Scholar]
  3. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacteriocins. Bacteriological Reviews 31:230–314
    [Google Scholar]
  4. Gillies R. R., Govan J. R. W. 1966; Typing of Pseudomonas pyocyanea by pyocine production. Journal of Pathology and Bacteriology 91:339–345
    [Google Scholar]
  5. Govan J. R. W. 1968 The pyocines of Pseudomonas pyocyanea Ph.D. Thesis University of Edinburgh:
    [Google Scholar]
  6. Govan J. R. W. 1974; Studies on the pyocins of Pseudomonas aeruginosa: morphology and mode of action of contractile pyocins. Journal of General Microbiology 80:1–15
    [Google Scholar]
  7. Govan J. R. W., Gillies R. R. 1969; Further studies in the pyocine typing of Pseudomonas pyocyanea. Journal of Medical Microbiology 2:17–25
    [Google Scholar]
  8. Higerd T. B., Baechler C. A., Berk R. S. 1967; In vitro and in vivo characterization of pyocin. Journal of Bacteriology 93:1976–1986
    [Google Scholar]
  9. Ishii S., Nishi Y., Egami F. 1965; The fine structure of a pyocin. Journal of Molecular Biology 13:428–431
    [Google Scholar]
  10. Ito S., Kageyama M., Egami F. 1970; Isolation and characterization of pyocins from several strains of Pseudomonas aeruginosa. Journal of General and Applied Microbiology 16:205–214
    [Google Scholar]
  11. Kageyama M. 1970a; Genetic mapping of a bacteriocinogenic factor in Pseudomonas aeruginosa. I. Mapping of pyocin R2 factor by conjugation. Journal of General and Applied Microbiology 16:523–530
    [Google Scholar]
  12. Kageyama M. 1970b; Genetic mapping of a bacteriocinogenic factor in Pseudomonas aeruginosa. II. Mapping of pyocin R2 factor by transduction with phage F116. Journal of General and Applied Microbiology 16:531–535
    [Google Scholar]
  13. Kohn J. 1966; Modified procedure for pyocine typing. Journal of Clinical Pathology 19:403
    [Google Scholar]
  14. MacPherson J. N., Gillies R. R. 1969; A note on bacteriocine typing techniques. Journal of Medical Microbiology 2:161–165
    [Google Scholar]
  15. Ouchterlony O. 1949; Antigen antibody reactions in gels. Arkiv för kemi, mineralogiochgeologi 26:1–48
    [Google Scholar]
  16. Slayter H. W., Holloway B. W., Hall C. E. 1964; The structure of phages B3, E79 and F116. Journal of Ultrastructure Research 11:274–281
    [Google Scholar]
  17. Takeya K., Minamishima Y., Amako K., Ohnishi Y. 1967; A small rod-shaped pyocin. Virology 31:166–168
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-80-1-17
Loading
/content/journal/micro/10.1099/00221287-80-1-17
Loading

Data & Media loading...

Most cited Most Cited RSS feed