1887

Abstract

SUMMARY: Six strains of were trained to grow in continued subculture on a medium composed of 99 volumes of 1 % casein hydrolysate + 1 volume of heart broth, the mixture containing 0·5 % (w/v) glucose. Poor growth of limited viability was obtained in a medium consisting of 1 % casein hydrolysate + 0·5% glucose (w/v) only. The addition of a wide variety of different amino-acids, fatty acids, growth factors and mineral salts to certain of the less complex media did not compensate for the partial or complete omission of the heart broth. Of the glucose supplied in relatively complex media about 40 % was utilized, and lactic acid equivalent to 30-60% of the glucose utilized appeared in the medium.

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/content/journal/micro/10.1099/00221287-8-3-464
1953-06-01
2022-01-18
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References

  1. Barker S. B., Summerson W. M. 1941; The colorimetric determination of lactic acid in biological material. J. biol. Chem 138:535
    [Google Scholar]
  2. Brewer J. H. 1940; Clear liquid mediums for the ‘aerobic’ cultivation of anaerobes. J. Amer. med. Ass 115:598
    [Google Scholar]
  3. Clark W. M. 1928 The determination of hydrogen ions, 3rd ed. p. 210 London: Baillière, Tindall and Cox;
    [Google Scholar]
  4. Cole S. W., Onslow H. 1916; On a substitute for peptone and a standard nutrient medium for bacteriological purposes. Lancet ii:9
    [Google Scholar]
  5. Consden R., Gordon A. H., Martin A. J. P. 1944; Qualitative analysis of proteins: a partition chromatographic method using paper. Biochem. J 38:736
    [Google Scholar]
  6. Erikson D. 1940; Pathogenic anaerobes of the Actinomyces group. Spec. Rep. Ser. med. Res. Coun., Lond240
    [Google Scholar]
  7. Holm P. 1950; Studies on the aetiology of human actinomycosis. 1. The ‘other microbes’ of actinomycosis and their importance. Acta path, microbiol. scand 28:736
    [Google Scholar]
  8. Mackie T. J., McCartney J. E. 1938 Handbook of practical bacteriology, 5th ed. p. 93 Edinburgh: E. and S. Livingstone;
    [Google Scholar]
  9. Nelson N. 1944; A photometric adaptation of the Somogyi method for the determination of glucose. J. biol. Chem 153:375
    [Google Scholar]
  10. Rosebury T. 1944; The parasitic aetinomycetes and other filamentous microorganisms of the mouth. A review of their characteristics and relationships, of the bacteriology of actinomycosis, and of salivary calculus in man. Bact. Rev 8:189
    [Google Scholar]
  11. Thompson L. 1950; Isolation and comparison of Actinomyces from human and bovine infections. Proc. Mayo Clin 25:81
    [Google Scholar]
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