SUMMARY: Six strains of were trained to grow in continued subculture on a medium composed of 99 volumes of 1 % casein hydrolysate + 1 volume of heart broth, the mixture containing 0.5 % (w/v) glucose. Poor growth of limited viability was obtained in a medium consisting of 1 % casein hydrolysate + 0.5% glucose (w/v) only. The addition of a wide variety of different amino-acids, fatty acids, growth factors and mineral salts to certain of the less complex media did not compensate for the partial or complete omission of the heart broth. Of the glucose supplied in relatively complex media about 40 % was utilized, and lactic acid equivalent to 30-60% of the glucose utilized appeared in the medium.


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