1887

Abstract

Methods for the laboratory maintenance and purification of cultures of are detailed. The upper limit of temperature for the growth of the organism is 30°. Light exerts an augmenting effect on the production of the toxin by Rapid and economical procedures for the bioassay of the toxin are based on the use of Gambusia minnows or tadpoles. Cell-free centrifugates of the cultures contain heat-labile toxic material which is non-diffusible through cellophan, sensitive to oxidizing agents, and reversibly inactivated by mild acidity. The toxin is rapidly inactivated by ubiquitous bacterial species ( and ). Charcoals, clays and calcium sulphate are efficient adsorbents of the toxic material. The concentration levels of toxin in cultures of appear to reflect a dynamic equilibrium between toxin destruction and production; a similar equilibrium may prevail in nature.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-8-3-333
1953-06-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/8/3/mic-8-3-333.html?itemId=/content/journal/micro/10.1099/00221287-8-3-333&mimeType=html&fmt=ahah

References

  1. Ashworth C. T., Mason M. F. 1946; Observations on the pathological changes produced by a toxic substance in the blue-green algae (Microcystis aeruginosa). Amer. J. Path 22:369
    [Google Scholar]
  2. Chu S. P. 1942; The influence of the mineral composition of the medium on the growth of planktonic algae. I. Methods and culture media. J. Ecol 30:284
    [Google Scholar]
  3. Connell C. H., Gross J. B. 1950; Mass mortality of fish associated with the protozoan Gonyaulax in the Gulf of Mexico. Science 112:359
    [Google Scholar]
  4. Liebert F., Deerns W. M. 1920; Onderzoek naar de Oorzaak van een Visch- sterfte in den polder Workumer-Nieuwland, Nabij Workum. Verh. Rijksinst. VisschOnderz 1:81
    [Google Scholar]
  5. Otterstroem C. V., Steemann Nielsen E. 1939; Two cases of extensive mortality in fishes caused by the flagellate Prymnesium parvum Carter. Rep. Danish biol. Sta 44:5
    [Google Scholar]
  6. Prescott G. W. 1948; Objectionable algae, with reference to the killing of fish and other animals. Hydrobiologia 1:1
    [Google Scholar]
  7. Reich K., Aschner M. 1947; Mass development and control of the phyto-flagellate Prymnesium parvum in fishponds in Palestine. Palest. J. BotJerusalem 4:14
    [Google Scholar]
  8. Schreiman E., Rugh R. 1949; Effect of D.D.T. on functional development of larvae of Rana pipiens and Fundulus heteroclitus. Proc. Soc. exp. Biol., N.Y 70:431
    [Google Scholar]
  9. Shelubsky M. 1951; Observations on the properties of a toxin produced by Microcystis. Proc. Ont. Ass. theor. appl. Limnol 11:362
    [Google Scholar]
  10. Snieszko S. F., Griffin P. J., Friddle S. B. 1950; A new bacterium (Haemophilus piscium. n.sp.) from ulcer disease of trout. J. Bact 59:699
    [Google Scholar]
  11. Sommer H., Mounier R. P., Riegel B., Stranger D. W., Mold J. D., Wikholm P. M., Kiralis E. S. 1948a; Paralytic shellfish poison. I. Occurrence and concentration by ion exchange. J. Amer. chem. Soc 70:1015
    [Google Scholar]
  12. Sommer H., Riegel B., Stranger D. W., Mold J. D., Wikholm D. M., Mccaughey M. B. 1948b; Paralytic shellfish poison. II. Purification by chromatography. J. Amer. chem. Soc 70:1019
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-8-3-333
Loading
/content/journal/micro/10.1099/00221287-8-3-333
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error