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Volume 8, Issue 2

Chemotherapy and Plant Viruses Free

Abstract

SUMMARY: When the guanine analogue, 5-amino-7-hydroxy-1-v-triazolo () pyrimidine (guanazolo), was sprayed on the leaves of tobacco or plants it reduced the number of local lesions and delayed or inhibited systemic spread of lucerne mosaic virus. Guanazolo was more effective when applied before inoculation, but had some effect if applied up to about the second day after inoculation. The compound was more effective in solution in 0·1% sodium bicarbonate than in aqueous suspension. With mechanically inoculated plants guanazolo watered on the soil around the plants was less effective than when sprayed on the leaves.

Incubated with the virus the compound did not affect infectivity. In concentrations up to 0·005 guanazolo usually caused negligible plant damage, but at higher concentrations produced a slight yellowing and distortion in the younger leaves with general stunting if treatments were prolonged.

The virus-inhibitory activity of guanazolo was reversed by adenine, guanine and possibly by hypoxanthine, but not by xanthine, uric acid, theobromine, theophylline, caffeine, uracil or thymine.

The triazolo analogue of adenine severely damaged plants and had only slight virus inhibitory activity. The hypoxanthine analogue caused no plant damage. It was less effective than guanazolo in tobacco and but more effective in reducing the number of local lesions produced in beans. Thiouracil, methyl-thiouracil and propylthiouracil were ineffective against lucerne mosaic virus. Thiouracil caused fairly severe plant damage.

Guanazolo had slight or negligible effects on spotted wilt virus in tomato, potato viruses and in potato, and tobacco and pea mosaic virus in peas. Applied as a leaf spray at 0·01 concentration guanazolo delayed or prevented systemic movement of cucumber mosaic virus from mechanically inoculated cucumber leaves, but had no effect when watered on the soil. However, watering the compound on the soil gave some control when the virus was introduced by aphids.

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© Society for Gerenal Microbiology, 1953
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References

  1. Arnow P., Sampath A. C., Oleson J. J., Williams J. H. 1952; The effect of triazolo pyrimidine analogues on the growth of Stichococcussubtilis. Amer. J. Bot 39:151
     [Google Scholar]
  2. Beale H. P., Jones C. R. 1951; Virus diseases of tobacco mosaic and potato yellow dwarf not controlled by certain purified antibiotics. Contr. Boyce Thompson Inst 16, (8):395
     [Google Scholar]
  3. Commoner B., Mercer F. 1951; Inhibition of the biosynthesis of tobacco mosaic virus by thiouracil. Nature; Lond: 168113
     [Google Scholar]
  4. Fries N., Panders A. 1951; The growth inhibiting effect of 5-amino-7-hydroxytriazolo pyrimidine in fungi and its reversal by purines. Ark. Bot. 2nd Ser 1:5–437 Abstr. in Rev. appl. Mycol. 30, 339
    [Google Scholar]
  5. Fry P. R. 1953; The occurrence of lucerne mosaic virus in New Zealand. N.Z. J. Sei. Tech in the Press
    [Google Scholar]
  6. Kidder G. W., Dewey V. C. 1949; The biological activity of substituted purines. J. biol. Chem 179:181
     [Google Scholar]
  7. Kidder G. W., Dewey V. C., Parks R. E. Jun Woodside G. L. 1949; Purine metabolism in Tetrahymena and its relation to malignant cells in mice. Science 109:511
     [Google Scholar]
  8. Köhler E., Hauschild I. 1950; Versuche zur Beeinflussung blattrollkranker Kartoffelknollen durch Chemikalien. NachrBl. dtsch. PflSchDienst, Stuttgart 2:24 Abstr. in Rev. appl. Mycol. 30, 186
    [Google Scholar]
  9. Kutsky R. J., Rawlins T. E. 1950; Inhibition of virus multiplication by naphthalene acetic acid in tobacco tissue cultures as revealed by a spectro-photometric method. J. Bact 60: 763
     [Google Scholar]
  10. Limasset P., Levieil F., Sechet M. 1948; Influence d’une phytohormone de synthèse sur le développement des virus X et Y de la pomme de terre chez le tabac. C.R. Acad. Sci.Paris 227:643 Abstr. in Rev. appl. Mycol. 28, 85
    [Google Scholar]
  11. Locke S. B. 1948; Studies on the chemotherapy of potato virus diseases. Phytopathology 38:916
     [Google Scholar]
  12. Manil P. 1947; Action negative, sur le virus de la mosaique du tabac, de la pénicilline et du prontosil. Ann. Inst. Pasteur 73:294
     [Google Scholar]
  13. Markham R., Smith J. D. 1949; Chromatography of nucleic acid derivatives. Nature; Lond: 163250
     [Google Scholar]
  14. Markham R., Smith K. M. 1949; Studies on the virus of turnip yellow mosaic. Parasitology 39:330
     [Google Scholar]
  15. Markham R., Smith J. D. 1951; Structure of Ribonucleic acid. Nature; Lond: 168406
     [Google Scholar]
  16. Marshak A., Vogel H. J. 1951; Microdetermination of purines and pyrimidines in biological materials. J. biol. Chem 189:597
     [Google Scholar]
  17. Matthews R. E. F. 1951; Effect of some substituted purines on the development of plant virus infections. Nature; Lond: 167892
     [Google Scholar]
  18. Matthews R. E. F. 1952; Effect of purines on the multiplication of plant viruses. Nature; Lond: 169500
     [Google Scholar]
  19. Nickell L. G. 1951; Effect of certain dyes on the growth in vitro of virus tumor tissue from Rumex acetosa. Bot. Gaz 112:290
     [Google Scholar]
  20. Nickell L. G., Greenfield P., Burkholder P. R. 1950; Atypical growth of plants. III. Growth responses of virus tumors of Rumex to certain nucleic acid components and related compounds. Bot. Gaz 112:42
     [Google Scholar]
  21. Roblin R. O. Jr Lampen J. O., English J. P., Cole Q. P., Vaughan J. R. Jr 1945; Studies in chemotherapy. VIII. Methionine and purine antagonists and their relation to the sulphonamides. J. Amer. chem. Soc 67:290
     [Google Scholar]
  22. Ryjkoff V. L., Smirnova V. A. 1948; The effect of magnesium ions and acridine preparations on the accumulation of virus proteins in plants affected with tobacco mosaic virus. Microbiology, Moscow 17:3 pp. 267 Abstr. in Rev. appl. Mycol. 28, 549
    [Google Scholar]
  23. Stoddard E. M. 1947; The X disease of peach and its chemotherapy. Bull. Conn, agric. Exp. Sta506
     [Google Scholar]
  24. Takahashi W. N. 1948; The inhibition of virus increase by malachite green. Science 107:226
     [Google Scholar]
  25. Thomas W. D., Baker R. R. 1949; Chemical inactivation of the carnation mosaic in vivo. J. Colo.-Wyo. Acad. Sci 4:51 Abstr. in Rev. appl. Mycol. 29, 563
    [Google Scholar]
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Chemotherapy and Plant Viruses
Microbiology 8, 277 (1953); https://doi.org/10.1099/00221287-8-2-277
/content/journal/micro/10.1099/00221287-8-2-277
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