1887

Microbiology Society logo

Volume 8, Issue 2

Factors affecting the Activation of the Ornithine Apodecarboxylase of a Strain of Free

Abstract

SUMMARY: The strain of used (strain 30) possessed decarboxylases specific for -histidine, -lysine and -ornithine. The rate of decarboxylation increased after a lag period to a maximum value which was dependent on the concentration of pyridoxal. A number of factors was found to affect the rate of activation of the apoenzymes, or, it is thought, probably the rate of pyridoxal phosphate synthesis. Among these factors were: the presence in the test system of the specific substrate for the decarboxylase, the concentration of pyridoxal, and the pH value of the test system. Cells grown in medium deficient in both pyridoxin and folic acid had greatly decreased activation rates for ornithine apodecarboxylase, and pyridoxal phosphate synthesis was also decreased. The addition of folic acid to the system did not affect the rate of activation with folic acid-deficient cells. The addition of thymine to the test system increased the activation rate for folic acid-rich cells, but had no effect with folic acid-deficient cells; other purines were ineffective. It is concluded that some product of thymine metabolism which folic acid-deficient cells are unable to make is concerned with the biological phosphorylation of pyridoxal by this organism.

  • Received:
  • Published Online:
© Society for Gerenal Microbiology, 1953
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-8-2-238
1953-04-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/8/2/mic-8-2-238.html?itemId=/content/journal/micro/10.1099/00221287-8-2-238&mimeType=html&fmt=ahah

References

  1. Bellamy W. D., Gunsalus I. C. 1944a; Tyrosine decarboxylation by streptococci: growth requirements for active cell production. J. Bact 48:191
     [Google Scholar]
  2. Bellamy W. D., Gunsalus I. C. 1944b; The function of pyridoxine and pyridoxine derivatives in the decarboxylation of tyrosine. J. biol. Chem 155:557
     [Google Scholar]
  3. Bellamy W. D., Gunsalus I. C. 1945; Tyrosine decarboxylase. II. Pyridoxin deficient medium for apoenzyme production. J. Bact 50:95
     [Google Scholar]
  4. Epps H. M. R. 1944; Studies on bacterial amino-acid decarboxylases. 2. 1( – )-Tyrosine decarboxylase from Streptococcus faecalis. Biochem. J 38:242
     [Google Scholar]
  5. Franklin A. L., Stokstad E. L. R., Hoffman C. E., Belt M., Jukes T. H. 1949; Inhibition of growth of Escherichia coli by 4-aminopteroylglutamic acid and its reversal. J. Amer. chem. Soc 71:3549
     [Google Scholar]
  6. Green D. E., Leloir L. F., Nocito V. 1945; Transaminases. J. biol. Chem 161:559
     [Google Scholar]
  7. Jukes T. H., Broquist H. P., Stokstad E. L. R. 1950; Vitamin B12 and ‘Citrovorum Factor’ in the nutrition of Lactobacillus leishmannii and Leuconostoc citrovorum. Arch. Biochem 26:157
     [Google Scholar]
  8. Martin G. J., Beiler J. M. 1947; Inhibition of the action of dopa decarboxylase. Arch. Biochem 15:201
     [Google Scholar]
  9. Rodwell A. W. 1953a; The occurrence and distribution of amino-acid decarboxylases within the genus Lactobacillus. J. gen. Microbiol 8:224
     [Google Scholar]
  10. Rodwell A. W. 1953b; The histidine decarboxylase of a species of Lactobacillus; apparent dispensability of pyridoxal phosphate as coenzyme. J. gen. Microbiol 8:233
     [Google Scholar]
  11. Shive W., Eakin R. E., Harding W. M., Ravel J., Sutherland J. C. 1948; A crystalline factor functionally related to folic acid. J. Amer. chem. Soc 70:2299
     [Google Scholar]
  12. Sloane-Stanley G. H. 1949; The use of tyrosine apodecarboxylase of Streptococcus faecalis R for the estimation of codecarboxylase. Biochem. J 44:567
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-8-2-238
Loading
Factors affecting the Activation of the Ornithine Apodecarboxylase of a Strain of Lactobacillus
Microbiology 8, 238 (1953); https://doi.org/10.1099/00221287-8-2-238
/content/journal/micro/10.1099/00221287-8-2-238
/content/journal/micro/10.1099/00221287-8-2-238
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error