A Simple Method for Producing Microcultures in Hanging Drops with special reference to Organisms Utilizing Oils Free

Abstract

SUMMARY: An aseptic technique is described for obtaining small hanging drops on the surface of coverslips, using only apparatus that is cheap and easy to obtain. The technique has been used to demonstrate the growth of on liquid paraffin droplets.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-8-1-66
1953-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/8/1/mic-8-1-66.html?itemId=/content/journal/micro/10.1099/00221287-8-1-66&mimeType=html&fmt=ahah

References

  1. Erikson D. 1949; Differentiation of the vegetative and sporogenous phases of the actinomycetes. 4. The partially acid-fast proactinomycetes. J. gen. Microbiol 3:361
    [Google Scholar]
  2. Fleming A., Voureka A., Kramer I. R. H., Hughes W. H. 1950; The morphology and motility of Proteus vulgaris and other organisms cultured in the presence of penicillin. J. gen. Microbiol 4:257
    [Google Scholar]
  3. De Fonbrune P. 1948 Technique de Micromanipulation Paris: Masson et Cie;
    [Google Scholar]
  4. Gee A. H., Hunt G. A. 1928; Single cell techniques.A presentation of the pipette method as a routine laboratory procedure. J. Bact 16:327
    [Google Scholar]
  5. Gilbert P. T. Jun 1951; Silicone water-repellents for general use in the laboratory. Science 114:637
    [Google Scholar]
  6. Reyniers J. A. 1933; Studies in micrurgical technique. I. The adaption of single cell technique to routine use. J. Bact 26:251
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-8-1-66
Loading
/content/journal/micro/10.1099/00221287-8-1-66
Loading

Data & Media loading...

Most cited Most Cited RSS feed