The Effect of Physical and Chemical Changes on the Liberation of Phage Particles by Lysogenic Strains of Free

Abstract

SUMMARY: Phage lysis was induced in lysogenic cultures of by means of nitrogen mustard, mustard gas, sulphathiazole, glutathione, and sodium thiol-acetate.

The liberation of phage particles by these cultures was inhibited by urethane, ascorbic acid, higher concentrations of thiolacetate than that necessary to induce lysis, and by incubating either at 41–43·5° or at pH 5·5. Incubation at 22° in some cases also had a slight inhibitory. action.

The following substances had no effect on the liberation of phage particles by these cultures: penicillin, streptomycin, chloramphenicol, Proflavine, Rivanol, colchicine, methionine, cobalt sulphate, sodium citrate, brilliant green, sodium tetra-thionate, sodium taurocholate, sodium biselenite, casein digest and yeast extracts. Cultivation either in atmospheres containing different amounts of oxygen, or at 28°, or in media containing varying concentrations of sodium chloride was also without effect.

Cultures did not become non-lysogenic as a result of prolonged passage in broth containing either sodium citrate, ascorbic acid or urethane.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-8-1-116
1953-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/8/1/mic-8-1-116.html?itemId=/content/journal/micro/10.1099/00221287-8-1-116&mimeType=html&fmt=ahah

References

  1. Bertani G. 1951; Studies on lysogenesis. 1. The mode of phage liberation by lysogenic Escherichia coli.. J. Bad 62:293
    [Google Scholar]
  2. Boyd J. S. K. 1951; Observations on the relationship of symbiotic and lytic bacteriophage. J. Path. Bad 63:445
    [Google Scholar]
  3. Cavallo G. 1951; Induction de la lyse bacteriophagique de microbes lysogenes par le ‘shock’ thermique. C.R. Soc. Biol., Paris 145:1885
    [Google Scholar]
  4. Cavallo G., Cantelmo P. 1951; Induction par les rayons ultraviolet de la lyse bacteriophagique des staphylocoques lysogenes. C.R. Soc. Biol., Paris 145:1419
    [Google Scholar]
  5. Clarke N. A. 1952; Studies on the host-virus relationship in a lysogenic strain of Bacillus megaterium. II. The growth of Bacillus megaterium in a synthetic medium. J. Bad 63:187
    [Google Scholar]
  6. Clarke N. A., Cowles P. B. 1952; Studies on the host-virus relationship in a lysogenic strain of Bacillus megaterium. 1. The relationship between growth and bacteriophage production in cultures of Bacillus megaterium.. J. Bact 63:177
    [Google Scholar]
  7. Ionesco H. 1952; C.R. Acad. Sci., Paris (in the Press). Quoted by Jacob, F. (1952). Ann. Inst. Pasteur 82:433
    [Google Scholar]
  8. Jacob F. 1950; Induction de la lyse et de la production de bacteriophage chez un Pseudomonas pyocyanea lysogene. C.R. Acad. Sci., Paris 231:1585
    [Google Scholar]
  9. Latarjet R. 1951; Induction, par les rayons X, de la production d’un bacteriophage chez B. megaterium lysogene. Ann. Inst. Pasteur 81:389
    [Google Scholar]
  10. Lwoff A. 1952; Rôle des cations bivalents dans l’induction du developpement du prophage par les agents reducteurs. C.R. Acad. Sci., Paris 234:366
    [Google Scholar]
  11. Lwoff A., Gutman A. 1950; Reclierches sur un Bacillus megaterium lysogène. Ann. Inst.Pasteur 78:711
    [Google Scholar]
  12. Lwoff A., Siminovitch L. 1951; Induction par des substances reductrices de la production de bacteriophage chez un bacterie lysogene. C.R. Acad. Sci., Paris 231:190
    [Google Scholar]
  13. Lwoff A., Siminovitch L., Kjeldgaard N. 1950; Induction de la production de bactériophages chez un bactérie lysogène. Ann. Inst.Pasteur 79:815
    [Google Scholar]
  14. Miles A. A., Misra S. S. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb 46:82
    [Google Scholar]
  15. Quersin L. 1951; Lyse induite du bacille lysogène de Lisbonne. C.R. Soc. Biol., Paris 145:1425
    [Google Scholar]
  16. Rountree P. M. 1951; A complement-fixing antigen of B. coli bacteriophage T5: its behaviour during virus growth. Brit. J. exp. Path 32:341
    [Google Scholar]
  17. Weigle J. J., Delbrück M. 1951; Mutual exclusion between an infecting phage and a carried phage. J. Bact 62:301
    [Google Scholar]
  18. Williams Smith H. 1951; Some observations on lysogenic strains of Salmonella. J. gen. Microbiol 5:458
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-8-1-116
Loading
/content/journal/micro/10.1099/00221287-8-1-116
Loading

Data & Media loading...

Most cited Most Cited RSS feed