@article{mbs:/content/journal/micro/10.1099/00221287-79-2-331, author = "Hopwood, D. A. and Wright, Helen M.", title = "A Plasmid of Streptomyces coelicolor Carrying a Chromosomal Locus and its Inter-specific Transfer", journal= "Microbiology", year = "1973", volume = "79", number = "2", pages = "331-342", doi = "https://doi.org/10.1099/00221287-79-2-331", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-79-2-331", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: A strain of Streptomyces coelicolor a3(2) was selected in which the wild-type autonomous SCPi plasmid had acquired an insertion of a region of the host chromosome bearing the cysB locus. This strain, called an SCPi′ strain by analogy with F′ strains of Escherichia coli, donated the cysB + allele very efficiently to a cysB recipient. The Cys+ progeny of such crosses were heterozygotes that gave rise to Cys– segregants by plasmid loss. Other donor markers, not carried on the plasmid, were donated with much lower frequencies. The SCPi-cysB plasmid was also donated rather efficiently to a strain of Streptomyces lividans, previously shown to receive the wild-type SCPi plasmid. Transfer back from S. lividans to S. coelicolor a3(2) occurred at a very much lower frequency.", }