SUMMARY: A strain of 3(2) was selected in which the wild-type autonomous SCP plasmid had acquired an insertion of a region of the host chromosome bearing the locus. This strain, called an SCP' strain by analogy with F' strains of , donated the allele very efficiently to a recipient. The Cys progeny of such crosses were heterozygotes that gave rise to Cys segregants by plasmid loss. Other donor markers, not carried on the plasmid, were donated with much lower frequencies. The SCP- plasmid was also donated rather efficiently to a strain of , previously shown to receive the wild-type SCP plasmid. Transfer back from to 3(2) occurred at a very much lower frequency.


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