1887

Abstract

SUMMARY: Osmotically fragile protoplasts have been prepared by the action of lytic enzymes on the mycelium of and The yield of protoplasts, based on DNA content, was up to 18 %. Pretreatment of the mycelium with a thiol compound was necessary with Cephalosporium but not with Penicillium. Electron micrographs indicated that the protoplasts contained all the intracellular organelles of the mycelium and were bounded only by a cytoplasmic membrane. The metabolic activity of the protoplasts, as measured by their respiration, ability to maintain intracellular amino-acid pools, and antibiotic production, was similar to that of control mycelium. -Valine and --aminoadipic acid, which are precursors of penicillin N and cephalosporin C, were taken up and concentrated by the protoplasts of , although the latter transported -valine less rapidly than did the corresponding mycelium. Protoplasts of took up -valine but not --aminoadipic acid or its -ester. There was little or no transport of the corresponding -amino acids by protoplasts of either organism.

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1973-12-01
2021-10-18
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References

  1. Abraham E. P., Newton G. G. F. 1954; Synthesis of d-δ-amino-δ-carboxyvaleryIglycine (a degradation product of cephalosporin N) and of dl-δ-amino-5-carboxyvaleramide. Biochemical Journal 58:266–268
    [Google Scholar]
  2. Bruno G. A., Christian J. E. 1961; Correction for quenching associated with liquid scintillation counting. Analytical Chemistry 33:650–651
    [Google Scholar]
  3. Burton K. 1968; Determination of DNA concentration with diphenylamine. In Methods in Enzymology vol xiib pp 163–166 Edited by Grossman L., Moldave K. New York: Academic Press;
    [Google Scholar]
  4. Gailey F. B., Stefaniak J. J., Olson B. H., Johnson M. J. 1946; A comparison of penicillin-producing strains of Penicillium notatum-chrysogenum. Journal of Bacteriology 52:129–140
    [Google Scholar]
  5. García López M. D., Rodriguez Aguirre M. J., Uruburu F., Villanueva J. R. 1967; Characterization of the lytic Streptomyces ra as a new strain of Streptomyces venezulae. Antonie van Leeuwenhoek 33:315–323
    [Google Scholar]
  6. Gibson R. K., Peberdy J. F. 1972; Fine structure of protoplasts of Aspergillus nidulans. Journal of General Microbiology 72:529–538
    [Google Scholar]
  7. Ishihara H., Sasaki T., Shimura K. 1968; Biosynthesis of bacitracin. II. Incorporation of 14C-labelled amino acids into bacitracin by a cell-free preparation from Bacillus licheniformis. Biochimica et biophysica acta 166:496–504
    [Google Scholar]
  8. Jarvis F. G., Johnson M. J. 1947; The role of the constituents of synthetic media for penicillin production. Journal of the American Chemical Society 69:3010–3017
    [Google Scholar]
  9. Kellenberger E., Ryter A., Sechaud J. 1958; Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. Journal of Biophysical and Biochemical Cytology 4:671–678
    [Google Scholar]
  10. Loder P. B., Abraham E. P. 1971; Biosynthesis of peptides containing α-aminoadipic acid and cysteine in extracts of a Cephalosporium sp. Biochemical Journal 123:477–482
    [Google Scholar]
  11. Loder P. B., Abraham E. P., Newton G. G. F. 1969; Behaviour of α-aminoadipylcysteine and glutamylcysteine in the presence of intact and disrupted mycelium of a Cephalosporium sp. Biochemical Journal 112:389–396
    [Google Scholar]
  12. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  13. Marini F., Arnow P., Lampen J. O. 1961; The effects of monovalent cations on the inhibition of yeast metabolism by nystatin. Journal of General Microbiology 24:51–62
    [Google Scholar]
  14. McIlvaine T. C. 1921; A buffer solution for colorimetric comparison. Journal of Biological Chemistry 49:183–186
    [Google Scholar]
  15. Nickerson W. J., Falcone G. 1956; Enzymatic reduction of disulphide bonds in cell wall protein of baker’s yeast. Science, New York 124:318–319
    [Google Scholar]
  16. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  17. Shimura K., Sasaki T., Sugawara K. 1964; Biosynthesis of bacitracin. I. Formation of bacitracin by a subcellular fraction of Bacillus licheniformis. Biochimica et biophysica acta 86:46–55
    [Google Scholar]
  18. Smith B., Warren S. C., Newton G. G. F., Abraham E. P. 1967; Biosynthesis of penicillin N and cephalosporin C. Antibiotic production and other features of the metabolism of a Cephalosporium sp. Biochemical Journal 103:877–890
    [Google Scholar]
  19. Sommer A., Lewis M. J. 1971; Effect of dithiothreitol on yeast: sphaeroplast formation and invertase release. Journal of General Microbiology 68:327–335
    [Google Scholar]
  20. Spackman D. H., Stein W. H., Moore S. 1958; Automatic recording apparatus for use in the chromatography of amino acids. Analytical Chemistry 30:1190–1206
    [Google Scholar]
  21. Tardrew P. L., Johnson M. J. 1958; Sulphate utilization by penicillin-producing mutants of Penicillium chrysogenum. Journal of Bacteriology 76:400
    [Google Scholar]
  22. Troy F. A., Koffler H. 1969; The chemistry and molecular architecture of the cell wall of Penicillium chrysogenum. Journal of Biological Chemistry 224:5563–5576
    [Google Scholar]
  23. Warren S. C., Newton G. G. F., Abraham E. P. 1967; The role of valine in the biosynthesis of penicillin N and cephalosporin C by a Cephalosporium sp. Biochemical Journal 103:902
    [Google Scholar]
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