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SUMMARY: Respiration of Crithidia fasciculata was more than 90 % inhibited by 0.5 mm-KCN or by a 19:1, CO:O2 gas mixture. Low-temperature difference spectra indicated the presence of a-, b- and c-type cytochromes and several CO-reacting haemoproteins. Photochemical action spectra revealed that cytochrome a 3 and another component (with an α-band in the CO-liganded state at 570 nm) act as functional terminal oxidases; no evidence was obtained for the presence of a functional cytochrome o. Extracts of the organism contain a cyanide-sensitive and CO-insensitive cytochrome c peroxidase. The possible identity of the haemoprotein(s) reacting with CO to give a Soret absorption maximum at 418 nm and previously referred to as cytochrome o is discussed.
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