1887

Abstract

Summary: Mucopeptide synthesis by ATCC 8081 and CRD (a methicillin-requiring substrain) was measured by incorporation of radioactivity from C-labelled-alanine, -glutamate, -lysine and -aspartate into the mucopeptide fraction of organisms incubated in a solution inadequate to support growth. Teichoic acid synthesis was measured by incorporation of [P]HPO into a hot trichloroacetic acid soluble fraction from isolated walls.

Mucopeptide synthesis by both strains (as measured by incorporation of [I-C]glutamic acid) was unaffected by chloramphenicol but was sensitive to benzylpenicillin. The incorporation was resistant to methicillin in strain CRD (2 mg/ml gave 50 % inhibition) but not in parent organisms (120 μg/ml gave 50 % inhibition).

Strain CRD incorporated twenty times less [P]H PO into teichoic acid than did the parent strain. With either strain, benzylpenicillin (25 μg/ml) and -cyclo-serine (200 μg/ml) inhibited this synthesis, but methicillin (500 and 1000 μg/ml) did not.

Electron micrographs of strain CRD incubated for four hours with methicillin showed large deposits of wall material around septa; these were not seen in parent organisms.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-79-2-195
1973-12-01
2020-11-29
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-79-2-195
Loading

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error