1887

Abstract

SUMMARY: Free and membrane-bound ribosomes were isolated from wild-type containing the genetic backgrund Em 5297a at 6, 12, 18, 24, 36, 48 and 72 h of growth. Log phase began at 8 h and continued to the end of 24 h, the beginning of the stationary phase. The ratio of free to membrane-bound ribosomes remained relatively constant during log phase. During stationary phase, the ratio of free to bound ribosomes rose sharply because of a decrease in the concentration of bound ribosomes.

Free ribosomes are generally assumed to synthesize intracellular proteins, whereas bound ribosomes synthesize proteins intended for secretion. These changes in membrane-bound ribosomes can be correlated with the specific activity of wallbound invertase (an intracellular enzyme) of the same strain of . We suggest that this decrease in membrane-bound ribosomes during stationary phase is of physiological significance and occurs because of a decreasing synthesis of secreted proteins.

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1973-12-01
2021-10-24
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References

  1. Beadle G. W., Tatum E. L. 1945; Neurospora. II. Methods of producing and detecting mutants with nutritional requirements. American Journal of Botany 32:678–686
    [Google Scholar]
  2. Blobel G., Potter V. R. 1967a; Studies on free and membrane-bound ribosomes in rat liver. 1. Distribution as related to total cellular RNA. Journal of Molecular Biology 26:279–292
    [Google Scholar]
  3. Blobel G., Potter V. R. 1967b; Ribosomes in rat liver: An estimation of the percentage of free and membrane-bound ribosomes interacting with messenger RNA in vivo. Journal of Molecular Biology 28:539–542
    [Google Scholar]
  4. Burton K. 1956; A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of DNA. Biochemical Journal 62:315–323
    [Google Scholar]
  5. Chang P. L. Y., Trevithick J. R. 1972; Distribution of wall-bound invertase during the asexual life cycle of Neurospora crassa. Journal of General Microbiology 70:23–29
    [Google Scholar]
  6. Denton R. M., Randle P. J. 1967; Concentration of glycerides and phospholipid in rat heart and gastrocnemius muscle. Biochemical Journal 104:416–422
    [Google Scholar]
  7. Drucker H. 1972; Regulation of exocellular proteases in Neurospora crassa: Induction and repression of enzyme synthesis. Journal of Bacteriology 110:1041–1049
    [Google Scholar]
  8. Falvey A. K., Staehelin T. 1970; Structure and function of mammalian ribosomes. I. Isolation and characterization of active liver ribosomal subunits. Journal of Molecular Biology 53:1–19
    [Google Scholar]
  9. Gare R., Setterfield G. 1972; Changes in ribosomes of artichoke tuber slices during ‘aging’. Canadian Society of Plant Physiologists, Eastern Section Meeting p 5
    [Google Scholar]
  10. Hayashi T. T., Kazmierowski D. 1972; Changes in free and membrane-bound ribosomes in the rat liver with starvation. Biochemistry 11:2371–2378
    [Google Scholar]
  11. Hicks S. J., Drysdale J. W., Munro H. N. 1969; Preferential synthesis of ferritin and albumin by different populations of liver polysomes. Science, New York 164:584–585
    [Google Scholar]
  12. Klis R. M., Hak A. 1972; Wall-bound invertase activity in Convolvulus callus: Increase after subculturing, and paradoxical effects of actinomycin D, cycloheximide, and thienylalanine. Physiologia plantarum 26:364–368
    [Google Scholar]
  13. Layne E. 1957; Spectrophotometric and turbidometric methods for measuring protein. In Methods in Enzymology vol 5 pp 450–451 Edited by Colowick S., Kaplan N. New York: Academic Press;
    [Google Scholar]
  14. Lindberg O., Ernster L. 1956; Determination of organic phosphorus compounds by phosphate analysis. Methods in Biochemical Analysis 3:1–19
    [Google Scholar]
  15. Merchant D. J., Kahn R. H., Murphy W. H. 1964 Handbook of Cell and Organ Culture pp 162–166 Minneapolis: Burgess;
    [Google Scholar]
  16. Palade G. E., Siekevitz P. 1956; Liver microsomes: An integrated morphological and biochemical study. Journal of Biophysical and Biochemical Cytology 25:171–198
    [Google Scholar]
  17. Redman C. M. 1968; The synthesis of serum proteins on attached rather than free ribosomes of rat liver. Biochemical and Biophysical Research Communications 31:845–850
    [Google Scholar]
  18. Rolleston F. S., Mak D. 1973; The binding of polyribosomes to smooth and rough endoplasmic-reticulum membranes. Biochemical Journal 131:851–853
    [Google Scholar]
  19. Schneider W. C. 1957; Determination of nucleic acids in tissues by pentose analysis. In Methods in Enzymology vol 3 pp 680–682 Edited by Colowick S. P., Kaplan N. O. New York: Academic Press;
    [Google Scholar]
  20. Sottocasa G. L., Kuylenstierna G., Ernster L., Bergstrand A. 1967; An electron transport system associated with the outer membrane of liver mitochondria. Journal of Cell Biology 32:415–438
    [Google Scholar]
  21. Spirin A. S., Gavrilova L. L. 1968 The Ribosome New York: Springer-Verlag;
    [Google Scholar]
  22. Storck R. 1963; Characterization of ribosomes from Neurospora crassa. Biophysics Journal 3:1–9
    [Google Scholar]
  23. Takagi M., Ogata K. 1968; Direct evidence for albumin synthesis by membrane-bound polysomes in rat liver. Biochemical and Biophysical Research Communications 33:55–60
    [Google Scholar]
  24. Tanaka T., Ogata K. 1971; Preferential synthesis of arginase by free polysomes from rat liver. Journal of Biochemistry 70:693–697
    [Google Scholar]
  25. Williams D. J., Rabin B. R. 1969; The effects of Aflatoxin B1 and steroid hormones on polysome binding to microsomal membranes as measured by activity of an enzyme catalysing disulphide interchange. Federation of European Biochemical Societies’ Letters 4:103–107
    [Google Scholar]
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