Summary: Mitochondria of NRRL 1960 and two mutants, 25/111 and β, were not able to oxidize tricarboxylic-acid-cycle intermediates, either in the presence of ADP or cytochrome , or after disruption of mitochondrial membranes. Reduced pyridine nucleotides, in particular NADH, were the only substrates respired. In the presence of NADH without added ADP the oxygen uptake was from 14 to 55 ng-atom oxygen/min/mg protein, while with ADP it was from 21 to 130 ng-atom O/min/mg protein, with respiratory control ratios from 1.8 to 2.9 and ADP/O ratios from 0.8 to 1.8. The lowest consumption of oxygen was shown by the mutant 25/111 dissimilating nitrate as the source of nitrogen.

The cultures converted malate to itaconate and succinate, fumarate to itaconate and one unknown acid, and pyruvate to all the acids usually produced in glucose media. Succinate, citrate, lactate, propionate and acetate supported growth without acid production.

It is concluded that the synthesis of itaconate in is probably not via the tricarboxylic acid cycle.


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