1887

Abstract

Lyophilized bacteria lose their ability to form colonies on nutrient media upon exposure to air (Naylor & Smith, 1946; Lion & Bergmann, 1961), oxygen being the lethal factor (Lion & Bergmann, 1961; Lion, 1963). The ability to reproduce decreases with exposure time. The ‘death’ of freeze-dried bacteria exposed to oxygen (FDO bacteria) does not abolish their ability to synthesize DNA, RNA and protein (Novick, Israeli & Kohn, 1972). However, DNA synthesis ceases a short time after reconstitution and -galactosidase production is blocked, to be resumed only after a repair period.

In order to check whether protein and DNA synthetic mechanisms could function correctly under some other control than that of the cell, we investigated the ability of FDO bacteria to produce phage.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-79-1-159
1973-11-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/79/1/mic-79-1-159.html?itemId=/content/journal/micro/10.1099/00221287-79-1-159&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1959 Bacteriophages New York: Interscience Publishers;
    [Google Scholar]
  2. Anderson J. D., Cox C. S. 1967; Microbial survival. In Airborne Microbes pp 203–226 Microbiology Society;
    [Google Scholar]
  3. Cox C. S., Baldwin F. 1966; The use of phage to study causes of loss of viability of Escherichia coli in aerosols. Journal of General Microbiology 44:15–22
    [Google Scholar]
  4. Israeli E., Kohn A. 1972; Protection of lyophilized E. coli from oxygen by Colicin E 1 treatment. FEBS Letters 26:323–326
    [Google Scholar]
  5. Lion M. B. 1963; Quantitative aspects of the protection of freeze-dried Escherichia coli against the toxic effect of oxygen. Journal of General Microbiology 32:321–329
    [Google Scholar]
  6. Lion M. B., Bergmann E. D. 1961; The effect of oxygen on freeze-dried Escherichia coli. Journal of General Microbiology 24:191–200
    [Google Scholar]
  7. Naylor H. B., Smith P. A. 1946; Factors affecting the viability of Serratia marcescens during dehydration and storage. Journal of Bacteriology 52:565–573
    [Google Scholar]
  8. Novick O., Israeli E., Kohn A. 1972; Nucleic acid and protein synthesis in reconstituted lyophilized Escherichia coli exposed to air. Journal of Applied Bacteriology 35:185–191
    [Google Scholar]
  9. Webb M. 1953; Effects of magnesium on cellular division in bacteria. Science, New York 118:607–611
    [Google Scholar]
  10. Webb S. J., Dumasia M. D., Singh Bhortee J. 1965; Bound water, inositol, and the biosynthesis of temperate and virulent bacteriophages by air-dried Escherichia coli. Canadian Journal of Microbiology 11:141–150
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-79-1-159
Loading
/content/journal/micro/10.1099/00221287-79-1-159
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error