1887

Abstract

SUMMARY: Three phages, isolated from soil samples collected in different localities, produced turbid plaques with a characteristic morphology on A3(2). Surviving growth from the plaques was lysogenic: when isolated and purified it was resistant to lysis by the homologous phage and released phage at a low frequency during growth. The three phages were homoimmune. The chromosomal attachment site of one of the prophages, VP5, was mapped in a position between and - that is, in a region devoid of standard markers - on the circular linkage map of the host, by crossing defective lysogens with sensitive strains. The host range of VP5 was restricted; only certain strains belonging to the group were attacked. VP5 gave rise to clear-plaque mutants, incapable of lysogenizing the host, at a spontaneous frequency of 10, which was increased by u.v. irradiation and by hydroxylamine mutagenesis; the clear-plaque mutants were incapable of lysing strains carrying the wild-type prophage. Electron microscopy showed that VP5 belongs to group B of Bradley’s morphological classification.

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/content/journal/micro/10.1099/00221287-78-2-349
1973-10-01
2024-12-06
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References

  1. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacteriocins.. Bacteriological Reviews 31:230–314
    [Google Scholar]
  2. Delic V., Hopwood D. A., Friend E. J. 1970; Mutagenesis by A-methyl-A’-nitro-TV-nitrosoguanidine (NTG) in Pseudomonas aeruginosa.. Mutation Research 9:167–182
    [Google Scholar]
  3. Dowding J. E. 1972 Studies on bacteriophages of Streptomyces coelicolor Ph.D. Thesis, University of East Anglia; Norwich:
    [Google Scholar]
  4. Dowding J. E. 1973; Characterisation of a bacteriophage virulent for Streptomyces coelicolor A3(2).. Journa l of General Microbiology 76:163–176
    [Google Scholar]
  5. Hopwood D. A. 1959; Linkage and the mechanism of recombination in Pseudomonas aeruginosa.. Annals of the New York Academy of Sciences 81:887–898
    [Google Scholar]
  6. Hopwood D. A. 1966a; Nonrandom location of temperature-sensitive mutants on the linkage map of Streptomyces coelicolor.. Genetics 54:1169–1176
    [Google Scholar]
  7. Hopwood D. A. 1966b; Lack of constant genome ends in Streptomyces coelicolor.. Genetics 54:1177–1184
    [Google Scholar]
  8. Hopwood D. A. 1967; Genetic analysis and genome structure in Pseudomonas aeruginosa.. Bacteriological Reviews 31:373–403
    [Google Scholar]
  9. Hopwood D. A. 1972; Genetics of the actinomycetales. In Actinomycetales: characteristics and practical importance pp 131–153 Edited by Sykes G., Skinner F. A. London: Academic Press.;
    [Google Scholar]
  10. Hopwood D. A., Chater K. F., Dowding J. E., Vivian A. 1973; Advances in Streptomyces coelicolor genetics.. Bacteriological Reviews (in the Press)
    [Google Scholar]
  11. Hopwood D. A., Harold R. J., Vivian A., Ferguson H. M. 1969; A new kind of fertility variant in Pseudomonas aeruginosa.. Genetics 62:461–477
    [Google Scholar]
  12. Jacob F., Wollman E. L. 1956; Sur les processus de conjugaison et de recombinaison génétique chez Pseudomonas aeruginosa. coli. I. L’induction par conjugaison ou induction zygotique.. Annales de l’Institut Pasteur 91:486–510
    [Google Scholar]
  13. Jacob F., Wollman E. L. 1959; Lysogeny.. In The Viruses vol 2 pp 319–351 Edited by Burnet F., Stanley W. M. New York: Academic Press.;
    [Google Scholar]
  14. Lieb M. 1953; The establishment of lysogeny in Pseudomonas aeruginosa.. Journal of Bacteriology 65:642–651
    [Google Scholar]
  15. Lomovskaya N. D., Emeljanova L. K., Alikhanian S. I. 1971; The genetic location of prophage on the chromosome of Streptomyces coelicolor.. Genetics 68:341–347
    [Google Scholar]
  16. Lomovskaya N. D., Mkrtumian N. M., Gostimskaya N. L., Danilenko V. N. 1972; Characterization of temperate actinophage 0C31 isolated from Streptomyces coelicolor A3(2).. Journal of Virology 9:258–262
    [Google Scholar]
  17. Najfeld V. 1973 A search for nonsense suppressors in Streptomyces coelicolor M.Phil. Thesis, University of East Anglia; Norwich:
    [Google Scholar]
  18. Sermonti G. 1969 Genetics of Antibiotic-producing Microorganisms London: Wiley-Interscience.;
    [Google Scholar]
  19. Shirling E. B. 1956; Studies on relationships between actinophage and variation in Streptomyces. I. Morphology of phage production and lysis in a streptomycete carrying a temperate phage.. Virology 2:272–283
    [Google Scholar]
  20. Vivian A. 1971; Genetic control of fertility in Streptomyces coelicolor A3(2): plasmid involvement in the interconversion of uf and if strains.. Journal of General Microbiology 69:353–364
    [Google Scholar]
  21. Vivian A., Hopwood D. A. 1970; Genetic control of fertility in Streptomyces coelicolor A3(2): the if fertility type.. Journal of General Microbiology 64:101–117
    [Google Scholar]
  22. Welsch M. 1956; Evidence for the occurrence of true lysogeny among actinomycètes.. Virology 2703–704
    [Google Scholar]
  23. Welsch M. 1959; Lysogenicity in streptomycetes.. Annals of the New York Academy of Sciences 8I:974–993
    [Google Scholar]
  24. Welsch M., Minon A., Schônfeld J. K. 1955; Isolation of actinophages.. Experientia II:24–25
    [Google Scholar]
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