1887

Abstract

SUMMARY: Growth of 107 on -alkanes (C, C or a mixture) completely repressed formation of acetyl-CoA carboxylase and partially repressed the fatty acid synthetase complex. As all fatty acids must then be derived directly from the substrate no matter what its chain length, the yeast must be able to elongate even-chain acids (C to C) and modify, by unknown reactions, odd-chain acids to give even-chain acids. Short-term control of fatty acid biosynthesis appears to be by long-chain (C or C) fatty acyl-CoA esters feedback-inhibiting the activities of both acetyl-CoA carboxylase and fatty acid synthetase. -Alkanes, -alcohol, free fatty acids or C and C acyl-CoA esters, had little or no effects on these enzymes. Extracts from -alkane-grown yeast inhibited the carboxylase in extracts from glucose-grown yeast, the pattern of inhibition being similar to that observed with hexadecyl-CoA.

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1973-10-01
2024-04-16
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References

  1. Bell G. H. 1971; The action of monocarboxylic acids on Candida tropicalis growing on hydrocarbon substrates.. Antonie van Leeuwenhoek 37:385–400
    [Google Scholar]
  2. Bell G. H. 1973; The metabolism of long chain fatty acids and alcohols by Candida tropicalis an.. Saccharomyces carlsbergensis. Antonie van Leeuwenhoek 39:137–149
    [Google Scholar]
  3. Birnbaum J. 1970; Repression of acetyl coenzyme A carboxylase by unsaturated fatty acid: relationship to coenzyme repression.. Journal of Bacteriology 104:171–176
    [Google Scholar]
  4. Bortz W. M., Lynen F. 1963; The inhibition of acetyl coenzyme A carboxylase by long-chain acyl coenzyme A derivatives.. Biochemische Zeitschrift 337:505–509
    [Google Scholar]
  5. Burton D. N., Collins J. M., Kennan A. L., Porter J. W. 1969; The effects of nutritional and hormonal factors on the fatty acid synthetase level of rat liver.. Journal of Biological Chemistry 244:4510–4516
    [Google Scholar]
  6. Butterworth P. H. W., Guchhait R. B., Baum H., Olson E. B., Margolis S. A., Porter J. W. 1966; Relationship between nutritional status and fatty acid synthesis by microsomal and soluble enzymes of pigeon liver.. Archives of Biochemistry and Biophysics 116:453–457
    [Google Scholar]
  7. Chenouda M. S., Jwanny E. W. 1972; Utilization of hydrocarbons by micro-organisms; lipids and phospholipids of Candida lipolytica grown on hexadecane and on glucose media.. Journal of General and Applied Microbiology 18:181–188
    [Google Scholar]
  8. Dahlen J. V., Kennan A. L., Porter J. W. 1968; Effect of alloxan and portacaval shunt on the synthesis of fatty acids and sterols by rat liver.. Archives of Biochemistry and Biophysics 124:51–57
    [Google Scholar]
  9. Davis J. B. 1964; Microbial incorporation of fatty acids derived from n-alkanes into glyceiides and waxes.. Applied Microbiology 12:210–214
    [Google Scholar]
  10. Dorsey J. A., Porter J. W. 1968; The effect of palmityl coenzyme A on pigeon liver fatty acid synthetase.. Journal of Biological Chemistry 243:3512–3516
    [Google Scholar]
  11. Gill C. O., Ratledge C. 1972; Toxicity of n-alkanes, n-alk-i-enes, n-alkan-i-ols and n-alkyl-i-bromides towards yeasts.. Journal of General Microbiology 72:165–172
    [Google Scholar]
  12. Gill C. O., Ratledge C. 1973a; Inhibition of glucose assimilation and transport by n-decane and other n-alkanes in Candida 107.. Journal of General Microbiology 75:11–22
    [Google Scholar]
  13. Gill C. O., Ratledge C. 1973b; Control of fatty acid biosynthesis in an alkane utilizing yeast.. Journal of General Microbiology 75:xvi–xvii
    [Google Scholar]
  14. Goodridge A. G. 1972; Regulation of the activity of acetyl coenzyme A carboxylase by palmityl coenzyme A and citiate.. Journal of Biological Chemistry 247:6946–6952
    [Google Scholar]
  15. Guynn R. W., Veloso D., Veech R. L. 1972; The concentration of malonyl CoA and the control of fatty acid synthesis Pseudomonas aeruginosa.. Journal of Biological Chemistry 247:7325–7331
    [Google Scholar]
  16. Henderson T. O., McNeill J. J. 1966; The control of fatty acid synthesis in Pseudomonas aeruginosa.. Biochemical and Biophysical Research Communications 25:662–669
    [Google Scholar]
  17. Henderson T. O., McNeill J. J. 1967; Further studies on the control of fatty acid synthesis in Pseudomonas aeruginosa.. Bacteriology Proceedings134
    [Google Scholar]
  18. Hornei S., Kohler M., Weide H. 1972; Das Fettsäurespektrum eines Candida-Stammes nach Kultur auf n-Alkenen.. Zeitschrift für allgemeine Mikrobiologie 11:19–27
    [Google Scholar]
  19. Hug H., Fiechter A. 1973; Assimilation of hydrocarbons by Candida tropicalis. II. Fatty acid profiles from cells grown on substrates of different chain length.. Archiv für Mikrobiologie 88:87–96
    [Google Scholar]
  20. Hunter K., Rose A. H. 1971; Yeast lipids and membranes.. The Yeasts vol 2 pp 211–270 Edited by Rose A. H., Harrison J. S. New York and London: Academic Press.;
    [Google Scholar]
  21. Lane M. D., Moss J., Ryder E., Stoll E. 1971; Activation of acetyl-CoA carboxylase by tricarboxylic acids. In Advances in Enzyme Regulation vol 9 pp 237–251 Edited by Weber G. Oxford: Pergamon Press;
    [Google Scholar]
  22. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  23. Lust G., Lynen F. 1968; The inhibition of the fatty acid synthetase multienzyme complex of yeast by long-chain acyl-coenzyme A compounds.. European Journal of Biochemistry 7:68–72
    [Google Scholar]
  24. Lynen F. 1969; Yeast fatty acid synthetase. In Methods in Enzymology vol 14 pp 17–32 Edited by Lowenstein J. M. New York and London: Academic Press.;
    [Google Scholar]
  25. Majerus P. W., Kilburn E. 1969; Acetyl-CoA carboxylase. The roles of synthesis and degradation in regulation of enzyme levels in rat liver.. Journal of Biological Chemistry 244:6254–6262
    [Google Scholar]
  26. Marquis N. R., Francesconi R. P., Villee C. A. 1968; A role for carnitine and long-chain acylcarni- tine in the regulation of lipogenesis. In Advances in Enzyme Regulation vol 6 pp 31–56 Edited by Weber G. Oxford: Pergamon Press;
    [Google Scholar]
  27. Matsuhashi M. 1969; Acetyl-coenzyme A carboxylase from yeast. In Methods in Enzymology vol 14 pp 3–8 Edited by Lowenstein M. J. New York and London: Academic Press.;
    [Google Scholar]
  28. Mizuno M., Shimojima Y., Iguchi T., Takeda I., Senoh S. 1966; Fatty acid composition of hydrocarbon-assimilating yeasts.. Agricultural and Biological Chemistry 30:506–510
    [Google Scholar]
  29. Moss J., Lane M. D. 1972; Acetyl-coenzyme A carboxylase. III. Further studies on the relation of catalytic activity to polymeric state.. Journal of Biological Chemistry 247:4944–4951
    [Google Scholar]
  30. Numa S., Bortz W. M., Lynen F. 1965; Regulation of fatty acid synthesis at the acetyl-coenzyme A carboxylation step. In Advances in Enzyme Regulation vol 3 pp 407–423 Edited by Weber G. Oxford: Pergamon Press;
    [Google Scholar]
  31. Numa S., Ringlemann E., Lynen F. 1965; Zur Hemmung der Acetyl Coenzyme A Carboxylase durch Fettsaure-CoA-Verbindungen.. Biochemische Zeitschrift 343:243–257
    [Google Scholar]
  32. Rasmussen R. K., Klein H. P. 1968; Activation of fatty acid synthesis in cell-free extracts of Pseudomonas aeruginosa.. Journal of Bacteriology 95:157–161
    [Google Scholar]
  33. Ratledge C. 1968; Production of fatty acids and lipids by Candida sp. growing on a fraction of n-alkanes predominating in tridecane.. Biotechnology and Bioengineering 10:511–533
    [Google Scholar]
  34. Ratledge C. 1970; Microbial conversions of n-alkanes to obtain economical microbial fats and fatty acids.. Chemistry and Industry pp 843–854
    [Google Scholar]
  35. Schweizer E., Kuhn L., Castorph H. 1971; A new gene cluster in yeast: the fatty acid synthetase system.. Hoppe-Seyler’s Zeitschrift für physiologische Chemie 352:377–384
    [Google Scholar]
  36. Seubert W. 1960; S-Palmityl coenzyme A. In Biochemical Preparations vol 7 pp 80–83 Edited by Lardy H. A. New York and London: John Wiley & Sons Inc.;
    [Google Scholar]
  37. Simon E. J., Shemin D. 1953; Synthesis of acetyl-CoA.. Journal of the American Chemical Society 75:2520
    [Google Scholar]
  38. Sumper M., Lynen F. 1972; The multienzyme system of fatty acid biosynthesis.. Hoppe-Seyler’s Zeitschrift für physiologische Chemie 353:502
    [Google Scholar]
  39. Suomalainen H., Keränen A. J. A. 1968; The fatty acid composition of baker’s and brewer’s yeast.. Chemistry and Physics of Lipids 2:296–315
    [Google Scholar]
  40. Thorpe R. F., Ratledge C. 1972; Fatty acid distribution in triglycerides of yeasts grown on glucose or n-alkanes.. Journal of General Microbiology 72:151–163
    [Google Scholar]
  41. Trust T. J., Millis N. F. 1970; The isolation and characterization of alkane-oxidizing organisms and the effect of growth substrate on isocitric lyase.. Journal of General Microbiology 61:245–254
    [Google Scholar]
  42. Trams E. G., Brady R. O. 1960; The synthesis of malonyl-14C CoA.. Journal of the American Chemical Society 82:2972–2973
    [Google Scholar]
  43. Tubbs P. K., Garland P. B. 1964; Variation in tissue contents of CoA thioesters and possible metabolic implications.. Biochemical Journal 93:550–557
    [Google Scholar]
  44. Vagelos P. R. 1971; Regulation of fatty acid biosynthesis. In Current Topics in Cellular Regulation vol 4 pp 119–166 Edited by Horecker B. L., Stadtman E. R. New York and London: Academic Press.;
    [Google Scholar]
  45. Wakil S. J., Goldman J. K., Williamson I. P., Toomey R. E. 1966; Stimulation of fatty acid biosynthesis by phosphorylated sugars.. Proceedings of the National Academy of Sciences of the United States of America 55:880–887
    [Google Scholar]
  46. Weeks G., Wakil S. J. 1970; Studies on the control of fatty acid metabolism. II. The inhibition of fatty acid synthesis in Lactobacillus plantarum by exogenous fatty acid.. Journal of Biological Chemistry 245:1913–1921
    [Google Scholar]
  47. White D., Klein H. P. 1965; Factors affecting fatty acid synthesis in cell-free preparations from Pseudomonas aeruginosa.. Biochemical and Biophysical Research Communications 20:78–84
    [Google Scholar]
  48. White D., Klein H. P. 1966; Effects of a-glycerophosphate and of palmityl coenzyme Aon lipid synthesis in yeast extracts.. Journal of Bacteriology 91:1218–1223
    [Google Scholar]
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