
Full text loading...
SUMMARY: Growth of Candida 107 on n-alkanes (C10, C16 or a mixture) completely repressed formation of acetyl-CoA carboxylase and partially repressed the fatty acid synthetase complex. As all fatty acids must then be derived directly from the substrate no matter what its chain length, the yeast must be able to elongate even-chain acids (C10 to C14) and modify, by unknown reactions, odd-chain acids to give even-chain acids. Short-term control of fatty acid biosynthesis appears to be by long-chain (C16 or C18) fatty acyl-CoA esters feedback-inhibiting the activities of both acetyl-CoA carboxylase and fatty acid synthetase. n-Alkanes, n-alcohol, free fatty acids or C12 and C14 acyl-CoA esters, had little or no effects on these enzymes. Extracts from n-alkane-grown yeast inhibited the carboxylase in extracts from glucose-grown yeast, the pattern of inhibition being similar to that observed with hexadecyl-CoA.
Article metrics loading...
Full text loading...
References
Data & Media loading...