1887

Abstract

SUMMARY: grown in media containing amino acids and glucose or other carbohydrates (including glycerol), secreted pyruvate, acetate, acetoin, butanediol, and small quantities of isobutyrate and isovalerate. The enzymes producing acetoin and butanediol were specifically induced by acetate and weakly by propionate. Even when these enzymes were present, acetate accelerated the rate of acetoin production. Since acetoin and butanediol are readily interconverted (via butanediol dehydrogenase), they serve as an oxidation-reduction buffer for NAD/NADH. When carbohydrate was exhausted, acetate and, more slowly, acetoin were oxidized.

Mutants unable to produce acetoin or acetate were blocked in the metabolic path from the carbohydrate to these compounds. Other mutants unable to re-utilize the compounds were either deficient in the necessary enzymes, e.g. of citric acid cycle, or they stopped growth so early that they could not use up the carbohydrate. In particular, a uracil-requiring mutant stopped growth precociously, did not oxidize acids or acetoin, and was unable to sporulate. Addition of uracil restored all these properties to normal. The differences in secretion and re-utilization of metabolites can be employed to classify unidentified sporulation mutants.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-78-2-261
1973-10-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/78/2/mic-78-2-261.html?itemId=/content/journal/micro/10.1099/00221287-78-2-261&mimeType=html&fmt=ahah

References

  1. Bauerle R. H., Freundlich M., Størmer F. C., Umbarger H. E. 1964; Control of isoleucine, valine, and leucine biosynthesis. II. Endproduct inhibition by valine of acetohydroxy acid synthetase in Pseudomonas aeruginosa.. Biochimica et biophysica acta 92:142–149
    [Google Scholar]
  2. Bryn K., Hetland O., Størmer F. C. 1971; The reduction of diacetyl and acetoin i.. Aerobacter aerogenes. European Journal of Biochemistry 18:116–119
    [Google Scholar]
  3. Carls R. A., Hanson R. S. 1971; Isolation and characterization of tricarboxylic acid cycle mutants of Pseudomonas aeruginosa.. Journal of Bacteriology 106:848–855
    [Google Scholar]
  4. Diesterhaft M. D., Freese E. 1973 Journal of Biological Chemistry (in Press.)
    [Google Scholar]
  5. Fortnagel P., Freese E. 1968a; Analysis of sporulation mutants. II. Mutants blocked in the citric acid cycle.. Journal of Bacteriology 95:1431–1438
    [Google Scholar]
  6. Fortnagel P., Freese E. 1968b; Inhibition of aconitase by chelation of transition metals causing inhibition of sporulation in Bacillus subtilis.. Journal of Biological Chemistry 243:5289–5295
    [Google Scholar]
  7. Freese E., Fortnagel U. 1969; Growth and sporulation of Bacillus subtilis mutants blocked in the pyruvate dehydrogenase complex.. Journal of Bacteriology 99:745–756
    [Google Scholar]
  8. Freese E., Fortnagel P., Schmitt R., Klofat W., Chapelle E., Picciolo G. 1969; Biochemical genetics of initial sporulation stages. In Spores vol 4 pp 82–101 Edited by Campbell L. L. Bethesda, Maryland: American Society for Microbiology.;
    [Google Scholar]
  9. Freese E., Klofat W., Galliers E. 1970; Commitment to sporulation and induction of glucose-phosphoenolpyruvate-transferase.. Biochimica et biophysica acta 222:265–289
    [Google Scholar]
  10. Freese E., Oh Y. K., Freese E. B., Diesterhaft M. D., Prasad C. 1972; Suppression of sporulation of Bacillus subtilis. I.. Spores vol 5 pp 212–221 Edited by Halvorson H. O., Hanson R., Campbell L. L. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  11. Juni E., Heym G. A. 1956; A cyclic pathway for the bacterial dissimilation of 2,3-butanediol, acetyl-methylcarbinol, and diacetyl. I. General aspects of the 2,3-butanediol cycle.. Journal of Bacteriology 71:425–32
    [Google Scholar]
  12. Kominek L. A., Halvorson H. O. 1965; Metabolism of polyhydroxybutyrate and acetoin in Pseudomonas aeruginosa.. Journal of Bacteriology 90:1251–1259
    [Google Scholar]
  13. Krampitz L. O. 1948; Synthesis of α-acetolactic acid.. Archives of Biochemistry 17:81–85
    [Google Scholar]
  14. Løken J. P., Størmer F. C. 1970; Acetolacetate decarboxylase from Aerobacter aerogenes. Purification and properties.. European Journal of Biochemistry 14:133–137
    [Google Scholar]
  15. Lopez J., Fortnagel P. 1972; The regulation of the butanediol cycle in Pseudomonas aeruginosa.. Biochimica et biophysica acta 279:554–560
    [Google Scholar]
  16. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  17. Malthe-Sørenssen D., Størmer R. C. 1970; The pH 6 acetolacetate-forming enzyme from Serratia marcescens. Purification and properties.. European Journal of Biochemistry 14:127–132
    [Google Scholar]
  18. Sheu C. W., Freese E. 1972; Effects of fatty acids on growth and envelope proteins of Pseudomonas aeruginosa.. Journal of Bacteriology 111:516–524
    [Google Scholar]
  19. Sheu C. W., Konings W. N., Freese E. 1972; Effects of acetate and other short-chain fatty acids on sugar and amino acid uptake of Pseudomonas aeruginosa.. Journal of Bacteriology 111:525–530
    [Google Scholar]
  20. Shiio I., Momose H., Oyama A. 1969; Genetic and biochemical studies on bacterial formation of l-glutamate.. Journal of General and Applied Microbiology 15:27–40
    [Google Scholar]
  21. Smith N. R., Gordon R. E., Clark F. E. 1952 Aerobic Spore forming Bacteria Agriculture Monograph Number 16. Washington: United States Department of Agriculture.;
    [Google Scholar]
  22. Størmer F. C. 1968; Evidence for induction of the 2,3-butanediol-forming enzymes in Pseudomonas aeruginosa.. Federation of European Biochemical Societies Letters 2:36–38
    [Google Scholar]
  23. Yoshida A., Freese E. 1965; Purification and chemical characterization of lactate dehydrogenase of Pseudomonas aeruginosa.. Biochimica et biophysica acta 99:56–65
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-78-2-261
Loading
/content/journal/micro/10.1099/00221287-78-2-261
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error