SUMMARY: The growth responses of 33 strains of cellulolytic butyrivibrios, isolated from the rumens of sheep fed on low-protein teff hay diets, to various combinations of volatile fatty acids (VFA) have been determined. Propionate alone was generally retardatory to the acetate-utilizing strains whilst acetate was stimulatory. Branched-chain VFA were generally stimulatory to those strains on which propionate had a retardatory effect, and retardatory to those strains on which propionate had no effect. Propionate alone was generally stimulatory to the acetate-producing strains and the effect of acetate and branched-chain VFA was variable. The major nutrient requirements of a few selected acetate-utilizing and acetate-producing strains are presented. A chemically defined medium was formulated which permits as good growth as a rumen-fluid medium for the acetate-utilizing butyrivibrios, Ruminococcus albus and Cillobacterium cellulosolvens.
AllisonM. J.,
BryantM. P.,
DoetschR. N.1962; Studies on the metabolic function of branched-chain volatile fatty acids, growth factors for ruminococci. I. Incorporation of isovalerate into leucine.. Journal of Bacteriology 83:523–532
BlockR. J.,
MitchellH. H.1946-47; The correlation of the amino-acid composition of proteins with their nutritive value.. Nutrition Abstracts and Reviews 16:249–278
BryantM. P.,
DoetschR. N.1955; Factors necessary for the growth of Bacteroides succinogenes in the volatile acid fraction of rumen fluid.. Journal of Dairy Science 38:340–350
BryantM. P.,
RobinsonI. M.1963; Apparent incorporation of ammonia and amino-acid carbon during growth of selected species of ruminal bacteria.. Journal of Dairy Science 46:150–154
BryantM. P.,
RobinsonI. M.,
ChuH.1959; Observations on the nutrition of Bacteroides succinogenes – a ruminal cellulolytic bacterium.. Journal of Dairy Science 42:1831–1847
HerbertH.1961; The chemical composition of micro-organisms as a function of their environment.. Symposium of the Society for General Microbiology 11:391–414
KistnerA., KotzeJ. P.1973; Enzymes of intermediary metabolism of Butyrivibrio fibrisolvens and Ruminococcus albus grown under glucose limitation.. Canadian Journal of Microbiology (in the Press)
LathamM. J.,
SharpeM. E.,
SuttonJ. D.1971; The microbial flora of the rumen of cows fed hay and high cereal rations and its relationship to the rumen fermentation.. Journal of Applied Bacteriology 34:425–434
LeeH. C.,
MooreW. E. C.1959; Isolation and fermentation characteristics of strains of Butyrivibrio from ruminal ingesta.. Journal of Bacteriology 77:741–747
PorterJ. W. G.1961; Vitamin synthesis in the rumen. In Digestive Physiology and Nutrition of the Ruminant pp 226–234 Edited by
LewisD.
London: Butterworth;
RochéC.,
AlbertynJ., KistnerA.1973; A method of preparing washed suspensions of anaerobic bacteria for metabolic studies.. Laboratory Practice (in the Press)
ShaneB. S.,
GouwsL., KistnerA.1969; Cellulolytic bacteria occurring in the rumen of sheep conditioned to low-protein teff hay.. Journal of General Microbiology 55:445–457
van GylswykN. O.1970; The effect of supplementing a low-protein hay on the cellulolytic bacteria in the rumen of sheep and on the digestibility of cellulose and hemicellulose.. Journal of Agricultural Science, Cambridge 74:169–180
van GylswykN. O.,
HoffmanJ. P. L.1970; Characteristics of cellulolytic cillobacteria from the rumens of sheep fed teff (Eragrostis tef) hay diets.. Journal of General Microbiology 60:381–386
van GylswykN. O.,
LabuschagneJ. P. L.1971; Relative efficiency of pure cultures of different species of cellulolytic rumen bacteria in solubilizing cellulose Pseudomonas aeruginosa.. Journal of General Microbiology 66:109–113
van GylswykN. O.,
RocheC. E. G.1970; Characteristics of Ruminococcus and cellulolytic Butyribrio species from the rumens of sheep fed differently supplemented teff (Eragrostis tef) hay diets.. Journal of General Microbiology 64:11–17
WellerR. A.1957; The amino-acid composition of hydrolysates of microbial preparations from the rumen of sheep.. Australian Journal of Biological Science 10:384–389