1887

Abstract

SUMMARY: The growth responses of 33 strains of cellulolytic butyrivibrios, isolated from the rumens of sheep fed on low-protein teff hay diets, to various combinations of volatile fatty acids (VFA) have been determined. Propionate alone was generally retardatory to the acetate-utilizing strains whilst acetate was stimulatory. Branched-chain VFA were generally stimulatory to those strains on which propionate had a retardatory effect, and retardatory to those strains on which propionate had no effect. Propionate alone was generally stimulatory to the acetate-producing strains and the effect of acetate and branched-chain VFA was variable. The major nutrient requirements of a few selected acetate-utilizing and acetate-producing strains are presented. A chemically defined medium was formulated which permits as good growth as a rumen-fluid medium for the acetate-utilizing butyrivibrios, and .

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1973-10-01
2024-11-02
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References

  1. Allison M. J., Bryant M. P., Doetsch R. N. 1962; Studies on the metabolic function of branched-chain volatile fatty acids, growth factors for ruminococci. I. Incorporation of isovalerate into leucine.. Journal of Bacteriology 83:523–532
    [Google Scholar]
  2. Block R. J., Mitchell H. H. 1946-47; The correlation of the amino-acid composition of proteins with their nutritive value.. Nutrition Abstracts and Reviews 16:249–278
    [Google Scholar]
  3. Bryant M. P., Doetsch R. N. 1955; Factors necessary for the growth of Bacteroides succinogenes in the volatile acid fraction of rumen fluid.. Journal of Dairy Science 38:340–350
    [Google Scholar]
  4. Bryant M. P., Robinson I. M. 1962; Some nutritional characteristics of predominant culturable ruminal bacteria.. Journal of Bacteriology 84:605–614
    [Google Scholar]
  5. Bryant M. P., Robinson I. M. 1963; Apparent incorporation of ammonia and amino-acid carbon during growth of selected species of ruminal bacteria.. Journal of Dairy Science 46:150–154
    [Google Scholar]
  6. Bryant M. P., Robinson I. M., Chu H. 1959; Observations on the nutrition of Bacteroides succinogenes – a ruminal cellulolytic bacterium.. Journal of Dairy Science 42:1831–1847
    [Google Scholar]
  7. Bryant M. P., Small N. 1956; The anaerobic monotrichous butyric acid-producing curved rod-shaped bacteria of the rumen.. Journal of Bacteriology 72:16–21
    [Google Scholar]
  8. Gill J. W., King K. W. 1958; Nutritional characteristics of a Pseudomonas aeruginosa.. Journal of Bacteriology 75:666–673
    [Google Scholar]
  9. Herbert H. 1961; The chemical composition of micro-organisms as a function of their environment.. Symposium of the Society for General Microbiology 11:391–414
    [Google Scholar]
  10. Hill C. H. 1952; Studies on the inhibition of growth of Streptococcus faecalis by sodium propionate.. Journal of Biological Chemistry 199:329–332
    [Google Scholar]
  11. Hungate R. E. 1950; The anaerobic mesophilic cellulolytic bacteria.. Bacteriological Reviews 14:1–50
    [Google Scholar]
  12. Hungate R. E. 1957; Micro-organisms in the rumen of cattle fed a constant ration.. Canadian Journal of Microbiology 3:289–311
    [Google Scholar]
  13. Hungate R. E. 1966 The Rumen and its Microbes, 1st edn. New York and London: Academic Press;
    [Google Scholar]
  14. Hungate R. E., Philips G. D., McGregor A., Hungate D. P., Buechner H. K. 1959; Microbial fermentation in certain mammals.. Science, New York 130:1192–1194
    [Google Scholar]
  15. Kistner A., Kotze J. P. 1973; Enzymes of intermediary metabolism of Butyrivibrio fibrisolvens and Ruminococcus albus grown under glucose limitation.. Canadian Journal of Microbiology (in the Press)
    [Google Scholar]
  16. Latham M. J., Sharpe M. E., Sutton J. D. 1971; The microbial flora of the rumen of cows fed hay and high cereal rations and its relationship to the rumen fermentation.. Journal of Applied Bacteriology 34:425–434
    [Google Scholar]
  17. Lee H. C., Moore W. E. C. 1959; Isolation and fermentation characteristics of strains of Butyrivibrio from ruminal ingesta.. Journal of Bacteriology 77:741–747
    [Google Scholar]
  18. Pennington R. J. 1957; Some effects of propionate upon acetate metabolism in animal tissue slices.. Biochemical Journal 65:534–540
    [Google Scholar]
  19. Porter J. W. G. 1961; Vitamin synthesis in the rumen. In Digestive Physiology and Nutrition of the Ruminant pp 226–234 Edited by Lewis D. London: Butterworth;
    [Google Scholar]
  20. Roché C., Albertyn J., Kistner A. 1973; A method of preparing washed suspensions of anaerobic bacteria for metabolic studies.. Laboratory Practice (in the Press)
    [Google Scholar]
  21. Shane B. S. 1966 The Predominant Cellulolytic Rumen Bacteria of Sheep Fed Low-Protein Teff Hay Ph.D. Thesis, University of Witwatersrand;
    [Google Scholar]
  22. Shane B. S., Gouws L., Kistner A. 1969; Cellulolytic bacteria occurring in the rumen of sheep conditioned to low-protein teff hay.. Journal of General Microbiology 55:445–457
    [Google Scholar]
  23. van Gylswyk N. O. 1968 The Cellulolytic Rumen Bacteria of Sheep Fed Supplemented Hay Diets M.Sc. Thesis, University of Pretoria;
    [Google Scholar]
  24. van Gylswyk N. O. 1970; The effect of supplementing a low-protein hay on the cellulolytic bacteria in the rumen of sheep and on the digestibility of cellulose and hemicellulose.. Journal of Agricultural Science, Cambridge 74:169–180
    [Google Scholar]
  25. van Gylswyk N. O., Hoffman J. P. L. 1970; Characteristics of cellulolytic cillobacteria from the rumens of sheep fed teff (Eragrostis tef) hay diets.. Journal of General Microbiology 60:381–386
    [Google Scholar]
  26. van Gylswyk N. O., Labuschagne J. P. L. 1971; Relative efficiency of pure cultures of different species of cellulolytic rumen bacteria in solubilizing cellulose Pseudomonas aeruginosa.. Journal of General Microbiology 66:109–113
    [Google Scholar]
  27. van Gylswyk N. O., Roche C. E. G. 1970; Characteristics of Ruminococcus and cellulolytic Butyribrio species from the rumens of sheep fed differently supplemented teff (Eragrostis tef) hay diets.. Journal of General Microbiology 64:11–17
    [Google Scholar]
  28. Weller R. A. 1957; The amino-acid composition of hydrolysates of microbial preparations from the rumen of sheep.. Australian Journal of Biological Science 10:384–389
    [Google Scholar]
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