1887

Abstract

Summary: A study has been made in methanol- and ethanol-grown x of the specific activities of the following enzymes: serine hydroxymethyltransferase, serine-glyoxylate aminotransferase, hydroxypyruvate reductase, glycerate kinase, phosphopyruvate hydratase, phosphopyruvate carboxylase, serine dehydratase, malate dehydrogenase (decarboxylating), phosphopyruvate synthase, phosphopyruvate carboxykinase, glycerate phosphomutase, malate dehydrogenase, malate lyase (CoA acetylating-ATP cleaving), citrate synthase, aconitate hydratase, isocitrate lyase and malate synthase. It was concluded that during growth on ethanol the glyoxylate cycle operated, while during growth on methanol, C units and glyoxylate were converted to malate by the serine pathway. The glyoxylate was regenerated by cleavage of malate into glyoxylate and acetyl-CoA. The acetyl-CoA was itself oxidized to glyoxylate by some of the reactions of the tricarboxylic acid cycle and isocitrate lyase, thus permitting net synthesis of C and C compounds from methanol and carbon dioxide.

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/content/journal/micro/10.1099/00221287-78-1-155
1973-09-01
2024-04-20
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References

  1. Atkinson D. E. 1971 In Metabolic Pathways vol v, p 1 Edited by Henry J. Vogel. New York and London: Academic Press;
    [Google Scholar]
  2. Attwood M. M., Harder W. 1972; A rapid and specific enrichment procedure for Hyphomicrobium spp.. Antonie van Leeuwenhoek 38:369–378
    [Google Scholar]
  3. Attwood M. M., Harder W. 1973; The metabolism of organic carbon compounds in Hyphomicrobia: metabolism of C2-compounds.. Antonie van Leeuwenhoek (in the Press)
    [Google Scholar]
  4. Bellion E., Hersh L. B. 1972; Methylamine metabolism in a Pseudomonas species.. Archives of Biochemistry and Biophysics 153:368–374
    [Google Scholar]
  5. Blackmore M. A., Quayle J. R. 1970; Microbial growth on oxalate by a route not involving glyoxylate carboligase.. Biochemical Journal 118:53–59
    [Google Scholar]
  6. Cánovas J. L., Kornberg H. L. 1969; Phosphoenolpyruvate carboxylase from Escherichia coli. I.. Methods in Enzymology vol 13 pp 288–309 Edited by Lowenstein J. M. New York and London: Academic Press.;
    [Google Scholar]
  7. Cleland W. W., Thompson V. W., Barden R. E. 1969; Isocitrate dehydrogenase (TPN-specific) from pig heart. In Methods in Enzymology vol 13 pp 30–33 Edited by Lowenstein J. M. New York and London: Academic Press.;
    [Google Scholar]
  8. Cooper R. A., Kornberg H. L. 1969; Phosphoenolpyruvate synthase. In Methods in Enzymology vol 13 pp 309–314 Edited by Lowenstein J. M. New York and London: Academic Press.;
    [Google Scholar]
  9. Dixon G. H., Kornberg H. L. 1959; Assay methods for key enzymes of the glyoxylate cycle.. Biochemical Journal 72:3 P
    [Google Scholar]
  10. Dixon G. H., Kornberg H. L. 1969; Malate synthase from baker’s yeast. In Methods in Enzymology vol 5 pp 633–637 Edited by Colowick S. P., Kaplan N. O. New York and London: Academic Press.;
    [Google Scholar]
  11. Dunstan P. M., Anthony C., Drabble W. T. 1972; Microbial metabolism of C1 and C2 compounds. The role of glyoxylate, glycollate and acetate in the growth of Pseudomonas AM1 on ethanol and on C1 compounds.. Biochemical Journal 128:107–115
    [Google Scholar]
  12. Fansler B., Lowenstein J. M. 1969; Aconitase from pig heart. In Methods in Enzymology vol 13 pp 26–30 Edited by Lowenstein J. M. New York and London: Academic Press.;
    [Google Scholar]
  13. Harder W., Quayle J. R. 1971; Aspects of glycine and serine biosynthesis during growth of Pseudomonas am1 on C1 compounds.. Biochemical Journal 121:763–769
    [Google Scholar]
  14. Heptinstall J., Quayle J. R. 1970; Pathways leading to and from serine during growth of Pseudomonas am1 on C1 compounds or succinate.. Biochemical Journal 117:563–572
    [Google Scholar]
  15. Hersh L. B., Bellion E. 1972; Malate cleavage reaction in Pseudomonas species.. Biochemical and Biophysical Research Communications 48:712–719
    [Google Scholar]
  16. Hsu R. Y., Lardy H. A. 1969; Malic enzyme. In Methods in Enzymology vol 13 pp 230–235 Edited by Lowenstein J. M. New York and London: Academic Press.;
    [Google Scholar]
  17. Kornberg H. L. 1970; Mechanisms of carbohydrate synthesis from C2- and C3-acids. I.. Miami Winter Symposium vol 1 pp 103–121 Amsterdam: North-Holland Publishing Co.;
    [Google Scholar]
  18. Lamprecht W., Heinz F., Diamartstein T. 1962; Phosphorylierung von d-GIycerinsäure zu 2-Phospho-d-Glycerinsäure mit Glyceratkinase in Leber. Identifizierung des Reaktionsproduktes durch Papier- chromatographie.. Zeitschrift für Physiologische Chemie 328:204–206
    [Google Scholar]
  19. Large P. J., Peel D., Quayle J. R. 1961; Microbial growth on C1 compounds. 2. Synthesis of cell constituents by methanol- and formate-grown Pseudomonas AM1, and methanol-grow.. Hyphomicrobium vulgare. Biochemical Journal 81:470–480
    [Google Scholar]
  20. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  21. Quayle J. R. 1972; The metabolism of one-carbon compounds in micro-organisms. In Advances in Microbial Physiology vol 7 pp 119–203 Edited by Rose A. H., Tempest D. W. London and New York: Academic Press.;
    [Google Scholar]
  22. Salem A. R., Large P. J., Quayle J. R. 1972; Glycine formation during growth of Pseudomonas AM1on methanol and succinate.. Biochemical Journal 124:1203–1211
    [Google Scholar]
  23. Sanwal B. D. 1970; Allosteric controls of amphibolic pathways in bacteria.. Bacteriological Reviews 30:20–39
    [Google Scholar]
  24. Scrutton M. C., Utter M. F. 1969; The regulation of glycolysis and gluconeogenesis in animal tissues.. Annual Review of Biochemistry 37:249–302
    [Google Scholar]
  25. Seubert W., Weicker H. 1969; Pyruvate carboxylase from Pseudomonas. I.. Methods in Enzymology vol 13 pp 258–262 Edited by Lowenstein J. M. New York and London: Academic Press.;
    [Google Scholar]
  26. Srere P. A. 1969; Citrate synthase. In Methods in Enzymology vol 13 pp 3–11 Edited by Lowenstein J. M. New York and London: Academic Press.;
    [Google Scholar]
  27. Stadtman E. R. 1957; Preparation and assay of acyl coenzyme A and other thiol esters; use of hydroxyl- amine. In Methods in Enzymology vol 3 pp 931–941 Edited by Colowick S. P., Kaplan N. O. New York: Academic Press.;
    [Google Scholar]
  28. Tuboi S., Kikuchi G. 1963; Enzymic cleavage of malate to glyoxylate and acetyl-coenzyme A.. Journal of Biochemistry, Japan 53:364–373
    [Google Scholar]
  29. Wright J. A., Sanwal B. D. 1969; Regulatory mechanisms involving nicotinamide adenine nucleotides as allosteric effectors. II. Control of phosphoe/10/pyruvate carboxykinase.. Journal of Biological Chemistry 244:1838–1845
    [Google Scholar]
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