1887

Abstract

Summary: Fractions containing particulate-bound ribosomes were prepared from conidia during germination and shown to transfer amino acids from aminoacyl-tRNA into protein. Regardless of the stage during germination used to prepare [C]aminoacyl-tRNA, the transfer activity of the fraction from germinated conidia was greater than that from freshly harvested conidia. Peptide-chain elongation factors also appear not to alter during germination. It is concluded that the activation of protein synthesis early in germination does not result from changes associated with the formation of aminoacyl-tRNA, or from changes in the peptide-chain elongation factors. Additional experiments revealed alterations in the attachment of these particulate-bound ribosomes during germination, and a scheme is proposed which relates these observations to the possible events occurring at the initiation of germination.

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1973-09-01
2021-08-04
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