RT Journal Article SR Electronic(1) A1 Brooks, J. D. A1 Meers, J. L.YR 1973 T1 The Effect of Discontinuous Methanol Addition on the Growth of a Carbon-limited Culture of Pseudomonas JF Microbiology, VO 77 IS 2 SP 513 OP 519 DO https://doi.org/10.1099/00221287-77-2-513 PB Microbiology Society, SN 1465-2080, AB SUMMARY: As the interval between methanol additions to methanol-limited cultures of Pseudomonas methylotropha was increased beyond 20 s the yield of bacterial dry wt/g of methanol fell significantly. Discontinuous methanol additions also caused cycling in the values of a number of parameters, including pH, dissolved oxygen tension, CO2 production and amino acid pool concentration. It is suggested that after each addition of methanol a burst of growth occurred, followed by a period of starvation. These observations are discussed in the light of continuous culture practice and theory., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-77-2-513