1887

Abstract

SUMMARY: As the interval between methanol additions to methanol-limited cultures of was increased beyond 20 s the yield of bacterial dry wt/g of methanol fell significantly. Discontinuous methanol additions also caused cycling in the values of a number of parameters, including pH, dissolved oxygen tension, CO production and amino acid pool concentration. It is suggested that after each addition of methanol a burst of growth occurred, followed by a period of starvation. These observations are discussed in the light of continuous culture practice and theory.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-77-2-513
1973-08-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/77/2/mic-77-2-513.html?itemId=/content/journal/micro/10.1099/00221287-77-2-513&mimeType=html&fmt=ahah

References

  1. Brown C. M., MacDonald-Brown D., Meers. J. L. 1973; Inorganic nitrogen assimilation by micro-organisms. Advances in Microbial Physiology (in the Press)
    [Google Scholar]
  2. Dawson P. S. S. 1965; Continuous phased growth in a modified chemostat. Canadian Journal of Microbiology 11:893–905
    [Google Scholar]
  3. Dawson P. S. S. 1971; A pilot plant apparatus for continuous phased culture–some observations on oxygen usage by Candida utilis during the cell cycle. Biotechnology and Bioengineering 8:877–892
    [Google Scholar]
  4. Degn H., Harrison D. E. F. 1969; Theory of oscillations of respiration rate in continuous culture of Pseudomonas aeruginosa. Journal of Theoretical Biology 22:238–248
    [Google Scholar]
  5. Gilley J. W., Bungay H. R. 1967; Oscillatory growth rate responses of Pseudomonas aeruginosa. cerevisiae in continuous culture. Biotechnology and Bioengineering 9:617–622
    [Google Scholar]
  6. Goodwin B. C. 1969; Synchronisation of Escherichia coli in a chemostat by periodic phosphate feeding. European Journal of Biochemistry 10:511–514
    [Google Scholar]
  7. Hansche P. E. 1969; A theoretical basis for the entrainment of chemostat populations. Journal of Theoretical Biology 24:335–350
    [Google Scholar]
  8. Harrison D. E. F., Pirt S. J. 1967; The influence of dissolved oxygen concentration on the respiration and glucose metabolism of Klebsiella aerogenes during growth. Journal of General Microbiology 46:193–211
    [Google Scholar]
  9. Herbert D. 1958; Some principles of continuous culture. In Recent Progress in Microbiology p 381 VIIth International Congress for Microbiology;
    [Google Scholar]
  10. Koga & Humphrey 1967; Study of the dynamic behaviour of the chemostat system. Biotechnology and Bioengineering 9:375–387
    [Google Scholar]
  11. MacLennan D. G., Ousby J. C., Vasey R. B., Cotton N. T. 1971; The influence of dissolved oxygen on Pseudomonas ami grown on methanol in continuous culture. Journal of General Microbiology 69:395–404
    [Google Scholar]
  12. Mateles R. I., Ryu D. Y., Yasada T. 1965; Measurement of unsteady steady state growth of micro-organisms. Nature, London 208:263–265
    [Google Scholar]
  13. Meers J. L. 1971; Effect of dilution rate on the outcome of chemostat mixed culture experiments. Journal of General Microbiology 67:359–361
    [Google Scholar]
  14. Meers J. L. 1973; Growth of bacteria in mixed culture. In Critical Reviews in Microbiology 2:139–183
    [Google Scholar]
  15. Meers J. L., Brooks J. D. 1973; The effects of discontinuous methanol addition on the growth of a methanol-limited culture of Pseudomonas aeruginosa. Journal of General Microbiology (In the Press.)
    [Google Scholar]
  16. Monod J. 1942 Recherches sur la croissance des cultures bactériennes. Paris: Herman et cie;
    [Google Scholar]
  17. Nagai S., Nishizawa Y., Endo I., Aiba S. 1968; Response of a chemostatic culture of Azotobacter vinelandii to a delta type pulse in glucose. Journal of General and Applied Microbiology 14:121–134
    [Google Scholar]
  18. Regan D. L., Roper G. H., Moss F. J. 1971; Response of continuous cultures to stimuli in glucose feed rate and dilution rate. Biotechnology and Bioengineering 8:815–824
    [Google Scholar]
  19. Tempest D. W., Meers J. L., Brown C. M. 1970; Synthesis of glutamate in Aerobacter aerogenes by a hitherto unknown route. Biochemical Journal 117. 405–407
  20. Tempest D. W., Meers J. L., Brown C. M. 1971; Influence of environment on the content and composition of microbial free amino acid pools. Journal of General Microbiology 64:171–185
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-77-2-513
Loading
/content/journal/micro/10.1099/00221287-77-2-513
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error