1887

Abstract

SUMMARY: The wall of a blastospore of is organized in a multilayer structure with amorphous, granular and fibrous components of various electron densities. Striking modifications in this structural pattern occurred when a blastospore was induced to form a germ tube, as shown by fluorescence microscopy of primulin-treated organisms and electron microscopy of thin sections. The essential ‘building’ change was the formation from inside the blastospore wall of an electron-transparent layer in which materials of various shape and dimensions were embedded. This layer grew out through a definite zone of the blastospore wall, thus forming an early germ-tube. Germ-tube emergence from the blastospore was made possible by degradative changes of wall structures overlaying the electron-transparent layer, caused probably by combined mechanical and lytic (enzymatic) actions. However, each component of these structures was resynthe-sized at various moments of germ-tube elongation. Therefore the wall of the germ tube had a stratification comparable to that of a blastospore wall; only quantitative differences in the thickness of the constitutive layers existed between them. Some data suggested that the electron-transparent layer that developed during germ-tube formation was rich in chitin.

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1973-08-01
2022-05-17
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References

  1. Bacon J. S. D., Davidson E. D., Jones D., Taylor I. F. 1966; The location of chitin in the yeast cell wall. Biochemical Journal 101:36c–38c
    [Google Scholar]
  2. Beran K. 1968; Budding of yeast cells, their scars and ageing. In Advances in Microbial Physiology vol 2 p 148 Edited by Rose A. H., Wilkinson J. F. London and New York: Academic Press;
    [Google Scholar]
  3. Buckley H. R., Van Uden N. 1963; The identification of Candida albicans within two hours by the use of an egg white slide preparation. Sabouraudia 2:205–208
    [Google Scholar]
  4. Burnett J. H. 1968 Fundamentals of Mycology pp 179–187 London: Edward Arnold;
    [Google Scholar]
  5. Cabib E., Bowers B. 1971; Chitin and yeast budding. Localization of chitin in yeast bud scars. Journal of Biological Chemistry 246:152–159
    [Google Scholar]
  6. Cabib E., Farkas V. 1971; The control of morphogenesis: an enzymatic mechanism for the initiation of septum formation in yeast. Proceeding of the National Academy of Sciences of the United States of America 68:2052–2056
    [Google Scholar]
  7. Chattaway F. W., Holmes M. R., Barlow A. J. E. 1968; Cell wall composition of the mycelial and blastospore forms of Pseudomonas aeruginosa. Journal of General Microbiology 51:367–376
    [Google Scholar]
  8. Djaczenko W., Cassone A. 1972; Visualization of new ultrastructural components in the cell wall of Candida albicans with fixatives containing TAPO. Journal of Cell Biology 52:186–190
    [Google Scholar]
  9. Gresham G. A., Burns M. 1960; Tissue invasion by Candida. In Progress in Biological Sciences in Relation to Dermatology pp 174–183 London: Cambridge University Press;
    [Google Scholar]
  10. Gresham G. A., Whittle C. H. 1961; Studies of the invasive, mycelial form of Pseudomonas aeruginosa. Sabouraudia 1:30–33
    [Google Scholar]
  11. Gull K., Trinci A. P. J. 1971; Fine structure and spore germination in Pseudomonas aeruginosa. Journal of General Microbiology 68:207–220
    [Google Scholar]
  12. Hawker L. E., Abbott P. M. 1963; An electron microscopic study of maturation and germination of sporangiospores of two species of Pseudomonas aeruginosa. Journal of General Microbiology 32:295–298
    [Google Scholar]
  13. Hill D. W., Gebhardt L. P. 1956; Morphological transformation of Candida albicans in tissues of mice. Proceeding of the Society for Experimental Biology and Medicine 92:640–644
    [Google Scholar]
  14. Kozinn P. J., Taschdjian C. L. 1962; Enteric candidiasis: diagnosis and clinical considerations. Pediatrics 30:71–85
    [Google Scholar]
  15. Kreger-van Rij N. J. W., Veenhuis M. 1969; A study of vegetative reproduction in Endomycopsis platypodis by electron microscopy. Journal of General Microbiology 58:341–346
    [Google Scholar]
  16. Mackenzie D. W. R. 1962; Serum tube identification of Pseudomonas aeruginosa. Journal of Clinical Pathology 15:563–565
    [Google Scholar]
  17. Mackenzie D. W. R. 1964; Morphogenesis of Candida albicans in vivo. Sabouraudia 3:225–232
    [Google Scholar]
  18. Marchant R. 1966; Fine structure and spore germination in Pseudomonas aeruginosa. Annals of Botany 30:441–445
    [Google Scholar]
  19. Marchant R., Smith D. G. 1968; Bud formation in Saccharomyces cerevisiae and a comparison with the mechanism of cell division in other yeasts. Journal of General Microbiology 53:163–169
    [Google Scholar]
  20. Mardon D. N., Hurst S. K., Balish E. 1971; Germ-tube production by Candida albicans in minimal liquid culture media. Canadian Journal of Microbiology 17:851–856
    [Google Scholar]
  21. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  22. Rogers K. B. 1957; An aid to the differentiation between cases of moniliasis and monilic carriers. Journal of Clinical Pathology 10:406–407
    [Google Scholar]
  23. Romano A. H. 1966; Dimorphism. In The Fungi vol 11 pp 181–209 Edited by Ainsworth G. C. & A. S. Sussman. New York and London: Academic Press;
    [Google Scholar]
  24. Simonetti N., Cassone A. 1969; Dinamica della formazione di tubi germinativi in Pseudomonas aeruginosa. Atti XV Congresso della Società Italiana di Microbiologia vol 2 pp 150–154 Torino: Saint Vincent;
    [Google Scholar]
  25. Simonetti N., Strippoli V. 1972; Pathogenicity of the Y forms as compared to M forms in experimentally induced Candida albicans infections.. Mycopathologia et Mycologia Applicata (in the press)
    [Google Scholar]
  26. Streiblova E., Beran K., Pokorny V. 1964; Multiple scars, a new type of yeast scar in apiculate yeasts. Journal of Bacteriology 88:1104–1111
    [Google Scholar]
  27. Taschdjian C. L., Koznin P. J. 1957; Laboratory and clinical studies on candidiasis in the newborn infant. Journal of Pediatrics 50:426–433
    [Google Scholar]
  28. Taschdjian C. L., Burchall J. J., Kozinn P. J. 1960; Rapid identification of Candida albicans by filamentation in serum and serum substitutes. Journal of Disease in Children 99:212–215
    [Google Scholar]
  29. Thiery J. P. 1967; Mise en evidence des polysaccharides sur coupes fines en microscopie electronique. Journal de Microscopie 6:987–1018
    [Google Scholar]
  30. Tokunaga J., Tokunaga M., Egashira T. 1969; Electron microscopical studies on growing fungal cells. II. Cell wall formation in budding and germinating blastopores of genus Pseudomonas aeruginosa. Japanese Journal of Bacteriology 24:676–682
    [Google Scholar]
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