1887

Abstract

SUMMARY: Carotenoids were produced in the mycelial form of only after exposure to light of wavelength < 500 nm, and production occurred in three stages. The initial photoinduction required light and was temperature-independent. Both subsequent dark stages, consisting of a lag period and of actual synthesis of carotenoids, were temperature-dependent. All steps had an absolute requirement for oxygen. Filtrates from dark-grown cultures contained ribo-flavin, while lumichrome was present in filtrates from light-grown cultures. Intracellular riboflavin levels were the same in both. Concentrations of diphenyl-amine which inhibited carotenogensis in light-grown cultures also inhibited extracellular production of riboflavin by dark-grown organisms. Several compounds with known photomimetic properties were tested on dark-grown mycelia, but pigmentation was induced only in plate cultures containing p-hydroxymercuri-benzoate. These pigments had the appearance of carotenoids, but have not been chemically characterized.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-77-2-403
1973-08-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/77/2/mic-77-2-403.html?itemId=/content/journal/micro/10.1099/00221287-77-2-403&mimeType=html&fmt=ahah

References

  1. Batra P. P. 1967; Mechanism of photoinduced carotenoid synthesis. Induction of carotenoid synthesis by antimycin A in the absence of light in Pseudomonas aeruginosa. Journal of Biological Chemistry 42:5630–5635
    [Google Scholar]
  2. Batra P. P. 1972; Mechanism of light induced carotenoid synthesis in non-photosynthetic plants. In Photophysiology vol 6 pp 47–76 Edited by Giese A. C. New York: Academic Press;
    [Google Scholar]
  3. Batra P. P., Rilling H. C. 1964; On the mechanism of photoinduced carotenoid synthesis. Aspects of the photoinductive reaction. Archives of Biochemistry and Biophysics 107:475–492
    [Google Scholar]
  4. Berends W., Posthuma J., Sussenbach j. S., Mager H. I. X. 1966 In Flavins and Flavoproteins pp 22–36 Edited by Slater E. C. Amsterdam: Elsevier;
    [Google Scholar]
  5. Burchard R. P., Hendricks S. B. 1969; Action spectrum for carotenogenesis in Pseudomonas aeruginosa. Journal of Bacteriology 97:1165–1168
    [Google Scholar]
  6. Carlile M. J. 1962; Evidence for a flavoprotein photoreceptor Pseudomonas aeruginosa. Journal of General Microbiology 28:161–167
    [Google Scholar]
  7. Carlile M. J. 1970; The photoresponses of fungi. In The Photobiology of Microorganisms pp 309–344 Edited by Halldal P. London: Wiley, Interscience;
    [Google Scholar]
  8. Codner R. C., Platt B. C. 1959; Light-induced production of carotenoid pigments by Cephalosporia. Nature, London 184:741–742
    [Google Scholar]
  9. Crandall B. S. 1945; A new species of Cephalosporium causing Persimmon wilt. Mycologia 37:495–498
    [Google Scholar]
  10. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  11. Eberhard D., Rau W., Zehender C. 1961; Über den Einfluss des Lichts auf die Carotinoidbildung von Pseudomonas aeruginosa. Planta 56:302–308
    [Google Scholar]
  12. Hager A. 1970; Formation of maxima in the absorption spectra of carotenoids in the region around 370 nm. Consequences for the interpretation of certain action spectra. Planta 91:38–45
    [Google Scholar]
  13. Hager A., Perutz H. 1970; Changes in the light absorption of a carotenoid in an enzyme (de-epoxidase) - substrate (violaxanthin) –complex. Planta 93:314–322
    [Google Scholar]
  14. Hollomon D. W. 1966; Reducing compounds and the growth of Pseudomonas aeruginosa. Journal of General Microbiology 45:315–324
    [Google Scholar]
  15. Howes C. D., Batra P. P. 1970; Mechanism of photoinduced carotenoid synthesis. Further studies on the action spectrum and other aspects of carotenogenesis. Archives of Biochemistry and Biophysics 137:175–180
    [Google Scholar]
  16. Howes C. D., Batra P. P., Blakely C. F. 1969; Absolute requirement for oxygen during illumination for photoinduced carotenoid synthesis. Biochimica et biophysica acta 189:298–299
    [Google Scholar]
  17. Kilgour G. L., Felton S. P., Huennekens F. M. 1957; Paper chromatography of flavins and flavin nucleotides. Journal of the American Chemical Society 79:2254–2256
    [Google Scholar]
  18. Kieber R. J., Payne W. J., Appleton G. S. 1955; The sterol content of fungi. I. Methods for disrupting cells, extracting and determining sterols. Applied Microbiology 3:247–248
    [Google Scholar]
  19. McNutt W. S. 1954; The direct contribution of adenine to the biogenesis of riboflavin by Pseudomonas aeruginosa. Journal of Biological Chemistry 210:511–518
    [Google Scholar]
  20. Olson J. A., Knizley J. H. 1962; The effect of diphenylamine on carotenoid, sterol and fatty acid synthesis in Pseudomonas aeruginosa. Archives of Biochemistry and Biophysics 97:138–145
    [Google Scholar]
  21. Page R. M. 1956; Studies on the development of asexual reproductive structures in Pseudomonas aeruginosa. Mycologia 48:206–224
    [Google Scholar]
  22. Page R. M. 1968; Phototropism in fungi. In Photophysiology vol 3 pp 65–90 Edited by Giese A. C. New York: Academic Press;
    [Google Scholar]
  23. Rau W. 1967a; Untersuchungen uber die lichtabhangige Carotenoidsynthese. I. Das Wirkungsspecktrum von Fusarium aquaeductuum. Planta 72:14–28
    [Google Scholar]
  24. Rau W. 1967b; Untersuchungen über die lichtabhängige Carotenoidsynthese. II. Erstatz der Lichtinduk- tion durch Mercuribenzoat. Planta 75:263–277
    [Google Scholar]
  25. Rau W. 1969; Untersuchungen über die lichtabhängige Carotenoidsynthese. IV. Die Rolle des Sauerstoffs bei der Lichtinduktion. Planta 84:30–42
    [Google Scholar]
  26. Rau W., Lindemann I., Rau-Hund A. 1968; Untersuchungen über die lichtabhängige Carotenoidsynthese. III. Die Farbstoffbildung von Neurospora crassa in Submerskultur. Planta 80:309–316
    [Google Scholar]
  27. Rilling H. C. 1962; Photoinduction of carotenoid synthesis of a Mycobacterium sp. Biochimica et biophysica acta 60:584–596
    [Google Scholar]
  28. Rilling H. C. 1964; On the mechanism of photo-induction of carotenoid-synthesis. Biochimica et biophysica acta 76:464–475
    [Google Scholar]
  29. Rilling H. C. 1965; A study of inhibition of carotenoid synthesis. Archives of Biochemistry and Biophysics 110:39–46
    [Google Scholar]
  30. Schaeffer P. 1953; A black mutant of Neurospora crassa. Mode of action of the mutant allele and action of light on melanogenesis. Archives of Biochemistry and Biophysics 47:359–379
    [Google Scholar]
  31. Shanmugan K. T., Berger L. R. 1969; Mechanism of catalase induction in Pseudomonas aeruginosa. Archiv für Mikrobiologie 69:206–215
    [Google Scholar]
  32. Stoudt T. H., Foster J. W. 1954; The microbiological synthesis of ergosterol. I. Assay procedure. Applied Microbiology 2:385–387
    [Google Scholar]
  33. Strong F. M. 1955; Riboflavin, folic acid and biotin. In Modem Methods of Plant Analysis vol Iv pp 643–660 Edited by Paech K., Tracey M. V. Berlin: Springer-Verlag;
    [Google Scholar]
  34. Taber W. A., Siepmann R. 1965; Measurement of growth in liquid cultures of molds. Applied Microbiology 13:827–828
    [Google Scholar]
  35. Theimer R. R., Rau W. 1969; Mutants of Fusarium aquaeductuum lacking photoregulation of carotenoid synthesis. Biochimica et biophysica acta 177:180–181
    [Google Scholar]
  36. Theimer R. R., Rau W. 1970; Untersuchungen über die lichtabhängige Carotenoidsynthese. V. Aufhebung der Lichtinduktion durch Reduktionsmittel und Ersatz des Lichts durch Wasserstoffperoxid. Planta 92:129–137
    [Google Scholar]
  37. Trinci A. P. J., Banbury G. H. 1969; Effect of light on growth and carotenogenesis of the tall conidiophores of Pseudomonas aeruginosa. Transactions of the British Mycological Society 52:73–86
    [Google Scholar]
  38. Valadon L. R. G., Mummery R. S. 1971; Effect of light on nucleic acids, protein and carotenoids of Pseudomonas aeruginosa. Microbios 4:227–240
    [Google Scholar]
  39. Weinhold A. R., Hendrix F. F. 1963; Inhibition of fungi by culture media previously exposed to light. Phytopathology 53:1280–1284
    [Google Scholar]
  40. Wolf F. T., Kim Y. T., Jones A. 1960; Spectral studies on chrysogenin, a pigment produced by Pseudomonas aeruginosa. Physiologia plantarum 13:621–627
    [Google Scholar]
  41. Zalokar M. 1954; Studies on biosynthesis of carotenoids in Pseudomonas aeruginosa. Archives of Biochemistry 50:71–80
    [Google Scholar]
  42. Zalokar M. 1955; Biosynthesis of carotenoids in Neurospora. Action spectrum of photoactivation. Archives of Biochemistry 56:318–325
    [Google Scholar]
  43. Zalokar M. 1957; Variation in the production of carotenoids in Pseudomonas aeruginosa. Archives of Biochemistry and Biophysics 70:561–567
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-77-2-403
Loading
/content/journal/micro/10.1099/00221287-77-2-403
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error